BBa_K542002BBa_K542002 Version 1 (Component)Lumazine Synthase with Transciptional Terminator and pBAD (No Promoter)
BBa_J120001BBa_J120001 Version 1 (Component)DFFb (CAD)-coding sequence for H. sapien DNA fragmentation factor beta
CBPa-BpABBa_K2053001 Version 1 (Component)Fusion cellulose-binding domain of CBP-B domain of protein A
BBa_K1714000BBa_K1714000 Version 1 (Component)pBad/araC-RBS B0034-Ag43 thanatin as alpha domain-double terminator
BBa_K2130004BBa_K2130004 Version 1 (Component)Report plasmid with toxin ccdB gene and inducible BAD promoter
BBa_K1111003BBa_K1111003 Version 1 (Component)Cad Promoter induced by low pH and high lysine conentrations
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K091181BBa_K091181 Version 1 (Component)pBad-RBS-tetR-TT-placI/cI-RBS-lasR-TT
BBa_J70631BBa_J70631 Version 1 (Component)R0010-J70630, rbs and maltose binding protein added to J70423
BBa_K091177BBa_K091177 Version 1 (Component)pMnt/tetR-RBS-GFP-TT-pBAD-RBS-tetR-TT
BBa_K091178BBa_K091178 Version 1 (Component)pMnt/tetR-RBS-GFP-TT-pBAD-RBS-Mnt-TT
BBa_K091176BBa_K091176 Version 1 (Component)pBAD-RBS-Mnt-TT-pMnt/tetR-RBS-GFP-TT
BBa_K091175BBa_K091175 Version 1 (Component)pBAD-RBS-tetR-TT-pMnt/tetR-RBS-GFP-TT
BBa_K1875005BBa_K1875005 Version 1 (Component)A 20 bp target sequence and PAM to make guide operator 1
BBa_K1875006BBa_K1875006 Version 1 (Component)A 20 bp target sequence and PAM to make guide operator 8
BBa_K1875004BBa_K1875004 Version 1 (Component)A 20 bp target sequence and PAM to make guide operator 3
BBa_K1875007BBa_K1875007 Version 1 (Component)A 20 bp target sequence and PAM to make guide operator 13
BBa_K1639009BBa_K1639009 Version 1 (Component)LacI regulated DsRed with miR 26a and miR 375 binding sites
BBa_K323089BBa_K323089 Version 1 (Component)In vivo testing device for protein-DNA binding: part 2 (lacZ_DTER_pBAD_BsaI_DTER)
BBa_K2170110BBa_K2170110 Version 1 (Component)Intermediate of biotin binding receptor (CMV_BM40_BAP_NanoLuc_A3C5) in RFC[25] N-part
BBa_K1994041BBa_K1994041 Version 1 (Component)sgRNA with dCas9 binding site Sequence 3 with PP7 handle insert
BBa_K2170111BBa_K2170111 Version 1 (Component)Intermediate of biotin binding receptor (CMV_BM40_eMA) in RFC[25] N-part
BBa_K2170112BBa_K2170112 Version 1 (Component)Intermediate of biotin binding receptor (CMV_BM40_scAvidin) in RFC[25] N-part
BBa_K323088BBa_K323088 Version 1 (Component)In vivo testing device for protein-DNA binding: part 1 (DTER_pSYN_BbsI)
BBa_J64982BBa_J64982 Version 1 (Component)OmpR-P strong binding, regulatory region for Team Challenge 03-2007
BBa_J64866BBa_J64866 Version 1 (Component)Strong OmpR Binding Operon for Team Challenge 03-2007
BBa_K2082298BBa_K2082298 Version 1 (Component)Modified lacZ promoter with OR1 and OR2 binding sites for cI of 434
BBa_K1371037BBa_K1371037 Version 1 (Component)pT7-RBS+loading module+KS-AT1+KR1+ACP+KS-AT2
BBa_J72106BBa_J72106 Version 1 (Component){araC-Pbad}{rbs.coi}{double terminator}{Ptet}{rbs.mRFP-3*}{double terminator}
CysE-sfGFPBBa_K731040 Version 1 (Component)Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE
BBa_K1114516BBa_K1114516 Version 1 (Component)Level 1 MoClo Device that expresses tetR under pBAD
BBa_K1114511BBa_K1114511 Version 1 (Component)Level 1 MoClo device that expresses YFP under pBAD
BBa_K2066117BBa_K2066117 Version 1 (Component)Synthetic Enhancer: 2x TetO Binding Cassette + NRII on UNS
BBa_K524203BBa_K524203 Version 1 (Component)pSC101 heat sensitive origin of replication with deletion in par locus
BBa_K2027033BBa_K2027033 Version 1 (Component)BlitzenBlue-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027027BBa_K2027027 Version 1 (Component)CupidPink-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027042BBa_K2027042 Version 1 (Component)ScoogeOrange-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027035BBa_K2027035 Version 1 (Component)LeorOrange-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2180013BBa_K2180013 Version 1 (Component)Promoter + Zinc Operator - CzrA Repressor Binding Site + Blue Pigment + Terminator
BBa_K2027043BBa_K2027043 Version 1 (Component)SeraphinaPink-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027032BBa_K2027032 Version 1 (Component)DonnerMagenta-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027028BBa_K2027028 Version 1 (Component)PrancerPurple-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027031BBa_K2027031 Version 1 (Component)VirginiaViolet-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027029BBa_K2027029 Version 1 (Component)VixenPurple-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027041BBa_K2027041 Version 1 (Component)DreidelTeal-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027030BBa_K2027030 Version 1 (Component)TinselPurple-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
BBa_K2027034BBa_K2027034 Version 1 (Component)MaccabeePurple-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
CBDcex(T7)BBa_K863102 Version 1 (Component)Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
BBa_K633015BBa_K633015 Version 1 (Component)AraC+Pbad promoter+RBS+ Ompa&lpp signal peptide+Sacc
BBa_K395403BBa_K395403 Version 1 (Component)pBad-cI-pλ-lacI-lacI+pL-GFP