BBa_K242300
BBa_K242300 Version 1 (Component)
multi-Ler binding site +promoter 1

BBa_K1989037
BBa_K1989037 Version 1 (Component)
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide PhoA

BBa_K923005
BBa_K923005 Version 1 (Component)
Ribosome Binding Site in pET14b plasmid

BBa_J58008
BBa_J58008 Version 1 (Component)
Periplasmic binding protein that docks a vanillin molecule

BBa_J64863
BBa_J64863 Version 1 (Component)
Strong OmpR Binding Operon for Team Challenge 03-2007

BBa_K541715
BBa_K541715 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_I720010
BBa_I720010 Version 1 (Component)
Ara landing pad (pBBLP 8)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.