BBa_K1833011
BBa_K1833011 Version 1 (Component)
lac promoter -> Zinc finger 225 binding domain fused to immunogenic MUC1 peptide

BBa_K2066112
BBa_K2066112 Version 1 (Component)
Synthetic Enhancer Project: Ntr promoter driven NRII2302 (mut) on UNS Standard

BBa_J06911
BBa_J06911 Version 1 (Component)
LacI ts (mut 241) QPI regulated by lambda cI with EYFP

BBa_J06912
BBa_J06912 Version 1 (Component)
LacI ts (mut 265) QPI regulated by lambda cI with EYFP

BBa_J06902
BBa_J06902 Version 1 (Component)
LacI ts (mut 265) QPI regulated by lambda cI with mCherry

BBa_J06901
BBa_J06901 Version 1 (Component)
LacI ts (mut 241) QPI regulated by lambda cI with mCherry

BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

BBa_J06961
BBa_J06961 Version 1 (Component)
Lambda cI/LacI ts (mut 241) RS flip-flop with EYFP

BBa_J06962
BBa_J06962 Version 1 (Component)
Lambda cI/LacI ts (mut 265) RS flip-flop with EYFP

BBa_K1014999
BBa_K1014999 Version 1 (Component)
Biological proportional operational Mu-circuit(1)

BBa_K929200
BBa_K929200 Version 1 (Component)
N-terminal sortase motif linked to AAV2-VP2 with Myc-tag, kozak and CMV

BBa_J06612
BBa_J06612 Version 1 (Component)
Construction intermediate: LacI ts (mut 265) QPI with Lambda cI regulated promoter (R0051.J06801)

BBa_J06611
BBa_J06611 Version 1 (Component)
Construction intermediate: LacI ts (mut 241) QPI with Lambda cI regulated promoter (R0051.J06800)

BBa_K427008
BBa_K427008 Version 1 (Component)
Ter PBadweak Mu Mor Ter PM GFP cassette

BBa_K427007
BBa_K427007 Version 1 (Component)
Ter PBadweak Mu C Ter Pmom GFP cassette

BBa_K1633005
BBa_K1633005 Version 1 (Component)
MOR siRNA-3 (siRNA for mouse Mu opioid receptor)

BBa_K1633004
BBa_K1633004 Version 1 (Component)
MOR siRNA-2 (siRNA for mouse Mu opioid receptor)

BBa_K1633003
BBa_K1633003 Version 1 (Component)
MOR siRNA-1 (siRNA for mouse Mu opioid receptor)

BBa_K1633006
BBa_K1633006 Version 1 (Component)
MOR siRNA-4 (siRNA for mouse Mu opioid receptor)

Pc-RhlR-mC
BBa_K1157010 Version 1 (Component)
Pc-RhlR-mCherry

BBa_K112502
BBa_K112502 Version 1 (Component)
< myc > in BBb

BBa_K1965002
BBa_K1965002 Version 1 (Component)
P3:FAStm:HA:TRPC1:Myc

BBa_J119336
BBa_J119336 Version 1 (Component)
Ammeline Biosensor with Riboswitch M6C prime

BBa_K189060
BBa_K189060 Version 1 (Component)
Gp15 of Bacteriophage Mu

BBa_K1014015
BBa_K1014015 Version 1 (Component)
Biological proportional operational Mu-circuit(1)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.