BBa_K1963002BBa_K1963002 Version 1 (Component)A template for expression of sRNA fusions to the E. coli micC hairpin
Bm3R1BBa_K1401000 Version 1 (Component)TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')
BBa_M50056BBa_M50056 Version 1 (Component)PETase double mutant I208V and R90A full plasmid information
BBa_J70032BBa_J70032 Version 1 (Component)A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)
BBa_K1051900BBa_K1051900 Version 1 (Component)G1 phase magic with cell synchronization device and CRISPRi induced alternative splicing device.
BBa_K1088059BBa_K1088059 Version 1 (Component)GFP reporter with flexible linker at N-terminus for creation of GFP fusions
BBa_K1088052BBa_K1088052 Version 1 (Component)GFP reporter with flexible linker at N-terminus for creation of GFP fusions
BBa_J58009BBa_J58009 Version 1 (Component)Fusion protein Trg-EnvZ
BBa_K2013000BBa_K2013000 Version 1 (Component)RBS and MHETase
BBa_K351005BBa_K351005 Version 1 (Component)Normal Human FGF receptor with fission yeast surface signal peptide
BBa_K2123110BBa_K2123110 Version 1 (Component)Improved Mer Operon Device with novel regulated exponential promoter
BBa_K2123301BBa_K2123301 Version 1 (Component)Novel Synthetic Phytochelatin codon optimized for E. coli expression
BBa_K1648004BBa_K1648004 Version 1 (Component)Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane
SISO_CNBBa_K255002 Version 1 (Component)pTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator
SISO_LacIBBa_K255003 Version 1 (Component)pLac.lacI-cfp(fusion).terminator.pTet.yfp.terminator
BBa_K1582025BBa_K1582025 Version 1 (Component)Thc_Cut1+sJanus-m Fusion Protein
BBa_K331021BBa_K331021 Version 1 (Component)Catechol 2,3 Dioxygenase with Fusion Suffix
mCherryBBa_K105010 Version 1 (Component)mCherry, Yeast optimized for fusion proteins
BBa_K105008BBa_K105008 Version 1 (Component)EYFP, yeast optimized for fusion proteins
BBa_K1114003BBa_K1114003 Version 1 (Component)The MoClo format of BBa_J23103 with AB fusion sites.
BBa_K351004BBa_K351004 Version 1 (Component)Modified Human FGF receptor for MPT51 antigen with fission yeast surface signal peptide
BBa_I20293BBa_I20293 Version 1 (Component)Constitutive expression of GFP-LacZalpha fusion
BBa_K1413045BBa_K1413045 Version 1 (Component)A fusion of Universal Transposon Plasmid and pSB1C3
AraC_TEV-FBBa_K627008 Version 1 (Component)Fusion part of arabinose-inducible induction system and the TEV protease
ssTorA_CS-BBa_K627012 Version 1 (Component)Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase
BBa_K2123113BBa_K2123113 Version 1 (Component)Stationary phase promoter with downstream mer operator + RFP (K081014)
BBa_K371054BBa_K371054 Version 1 (Component)MPF(meta-prefix)+[GFP+10*GS+A] fusion protein+MSF(meta-suffix))
BBa_K774102BBa_K774102 Version 1 (Component)Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide usin
BBa_K2123115BBa_K2123115 Version 1 (Component)Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014
BBa_K2123114BBa_K2123114 Version 1 (Component)Stationary phase promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
BBa_K2123116BBa_K2123116 Version 1 (Component)Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.