BBa_I746325
BBa_I746325 Version 1 (Component)
GFP under control of PLL promoter

BBa_K808028
BBa_K808028 Version 1 (Component)
PhoA: signal sequence for peri plasmic expression

BBa_K1315003
BBa_K1315003 Version 1 (Component)
The <i>rpfG</i> gene from <i>Xanthomonas campestris</i>

BBa_M31951
BBa_M31951 Version 1 (Component)
part of Gene II of M13K07

BBa_K1165012
BBa_K1165012 Version 1 (Component)
Ethylene Synthesis Cassette (SAM Synthetase, ACC Oxidase, ACC Synthase) under the Control of pTet Pr

BBa_I13412
BBa_I13412 Version 1 (Component)
CFP/YFP intermediate for screening plasmid v2

Ph-s
BBa_K143015 Version 1 (Component)
Promoter hyper-spank for B. subtilis

BBa_K2074133
BBa_K2074133 Version 1 (Component)
pChlamy_3

BBa_K2122150
BBa_K2122150 Version 1 (Component)
Sequence for Gb3 Synthase

BBa_K090403
BBa_K090403 Version 1 (Component)
Gram-positive Shuttle Vector for Chromosomal Integration

Prefix-R
BBa_G1003 Version 1 (Component)
Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

Suffix-F
BBa_G1002 Version 1 (Component)
Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

BBa_J05215
BBa_J05215 Version 1 (Component)
Regulator for R1-CREBH

GFP
BBa_M45413 Version 1 (Component)
Coding sequence for a green fluorescence protein back translated for homo sapiens

BBa_K1315006
BBa_K1315006 Version 1 (Component)
The <i>manA</i> promoter from <i>Xanthomonas campestris</i>

BBa_K112247
BBa_K112247 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid

BBa_K112246
BBa_K112246 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid

BBa_K112248
BBa_K112248 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid

BBa_K2018032
BBa_K2018032 Version 1 (Component)
RBS for panK

BBa_K2018031
BBa_K2018031 Version 1 (Component)
Promoter for panKII

BBa_K759006
BBa_K759006 Version 1 (Component)
B0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2018022
BBa_K2018022 Version 1 (Component)
The gene encoding phasin

BB base ve
BBa_I51020 Version 1 (Component)
BioBrick base vector (for constructing pSB**5 vectors)

BBa_K1491023
BBa_K1491023 Version 1 (Component)
C-terminus of T7 RNA Polymerase

BBa_K855003
BBa_K855003 Version 1 (Component)
coding sequence for pvdQ with histag added to the C-terminal followed by stop codon

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_I714889
BBa_I714889 Version 1 (Component)
OR21 of PR and PRM

Screening
pSB1A10 Version 1 (Component)
Screening Plasmid v1.0

BBa_K382000
BBa_K382000 Version 1 (Component)
pORE Open Series Vector with BioBrick MCS (Basta resistance)

BBa_K812001
BBa_K812001 Version 1 (Component)
Biobricked pSC2+ plasmid with pElastase promoter for frog use

BBa_K909007
BBa_K909007 Version 1 (Component)
TetR DNA binding domain containing BamHI site for protein fusions

BBa_I13506
BBa_I13506 Version 1 (Component)
Screening plasmid intermediate

BBa_K641004
BBa_K641004 Version 1 (Component)
rbs for ToxR

BBa_J331018
BBa_J331018 Version 1 (Component)
RBS for hsp90

BBa_K2116999
BBa_K2116999 Version 1 (Component)
RBS for EsaR

BBa_K2116031
BBa_K2116031 Version 1 (Component)
RBS for sfGFP

BBa_K209432
BBa_K209432 Version 1 (Component)
pCEP4 with AarI A-D sites in MCS

BBa_K1778003
BBa_K1778003 Version 1 (Component)
RtTA-flag is a regulatory protein that can regulate the binding of tetracycline analogues.

G
BBa_G0011 Version 1 (Component)
The nucleotide G

C
BBa_G0010 Version 1 (Component)
The nucleotide C

BBa_I746322
BBa_I746322 Version 1 (Component)
GFP under control of PP promoter

BBa_K258102
BBa_K258102 Version 1 (Component)
PrtF domain of ABC transporter of Erwinia chrysanthemi

FG
BBa_K1442106 Version 1 (Component)
Forward GFP for human cells

BBa_K2116028
BBa_K2116028 Version 1 (Component)
bxb1 integrase under the control of nitric oxide and AHL responsive AND gate

BBa_K782048
BBa_K782048 Version 1 (Component)
HindIII/XhoI equivalent multicloning site for insertion of non standardized parts to BioBrick vector

BBa_K782050
BBa_K782050 Version 1 (Component)
BamHI/XbaI equivalent multicloning site for insertion of non standardized parts to BioBrick vectors