BBa_K1088058BBa_K1088058 Version 1 (Component)10 aa linker with BamHI restriction site and TEV recognition site
BBa_K1638006BBa_K1638006 Version 1 (Component)10 aa linker with BamHI restriction site and TEV recognition site
BBa_K1180003BBa_K1180003 Version 1 (Component)Fusion protein of lamp 2b and pre-s1 for liver targeting of the exosome
BBa_K806003BBa_K806003 Version 1 (Component)SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins
BBa_K411123BBa_K411123 Version 1 (Component)Split RFP-eIF4A_A Generator (R0040 + B0034 + RFP_split_A+Linker+eIF4A_split_A + B0015)
pirABBa_K273002 Version 1 (Component)Pirin like protein (pirA) from <i>Synechocystis sp PCC6803</i>
BBa_K273003BBa_K273003 Version 1 (Component)Pirin like protein (pirB) from <i>Synechocystis sp PCC6803</i>
BBa_K195203BBa_K195203 Version 1 (Component)RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator
BBa_J24822BBa_J24822 Version 1 (Component)Same as J24819 but with the error at the luc-terminator junction fixed
BBa_K299803BBa_K299803 Version 1 (Component)Gactag - like a biobrick scar but first T changed to G
BBa_K855006BBa_K855006 Version 1 (Component)pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site
BBa_K855005BBa_K855005 Version 1 (Component)pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site
BBa_K300079BBa_K300079 Version 1 (Component)Phasin (PhaP) - head domain - with flexible protein domain linker downstream
BBa_K1616004BBa_K1616004 Version 1 (Component)VVD linked to YN155 (YFP Nter split)
BBa_K887004BBa_K887004 Version 1 (Component)Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015
BBa_J119408BBa_J119408 Version 1 (Component)Pupp promoter mutant - Substitution of C and G to A at 28 and 30
BBa_K202004BBa_K202004 Version 1 (Component)Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 3
BBa_K323005BBa_K323005 Version 1 (Component)CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system
BBa_K323021BBa_K323021 Version 1 (Component)CMV ATG Gli1 link nCFP stop - part of mammalian split/FRET system
BBa_K1178000BBa_K1178000 Version 1 (Component)tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon
IodoY RSBBa_K1416001 Version 1 (Component)The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon
BBa_K874999BBa_K874999 Version 1 (Component)Zinc Finger Array v1
BBa_K1361005BBa_K1361005 Version 1 (Component)CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive
BBa_K300096BBa_K300096 Version 1 (Component)Double phasin and intein separed by a flexible protein domain linker
BBa_K323029BBa_K323029 Version 1 (Component)CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system
BBa_K299106BBa_K299106 Version 1 (Component)xbaI G (xbaI site and additional G to behave like biobrick prefix after xba digest)
BBa_K351007BBa_K351007 Version 1 (Component)first Ig-like region of Human basic FGFR with signal peptide for s.pombe surface
BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
BBa_K1848001BBa_K1848001 Version 1 (Component)Human Gut Hormone Glucagon-like peptide 1 (7-37) (Sequence Lacks Stop Codon, but part possesses it)
BBa_K323075BBa_K323075 Version 1 (Component)ATG cYFP link HIVC
BBa_K1616003BBa_K1616003 Version 1 (Component)VVD link to YC155 (YFP Cter split)
BBa_K1961007BBa_K1961007 Version 1 (Component)CYP1A2, an enzyme of cytochrome P450 protein family that metabolizes toxin in liver
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.