BBa_M11407
BBa_M11407 Version 1 (Component)
Type 1 promoter of hspA gene in Synechocystis sp. PCC 6803

BBa_M11408
BBa_M11408 Version 1 (Component)
Type 1 promoter of sigA gene in Synechocystis sp. PCC 6803

BBa_M11404
BBa_M11404 Version 1 (Component)
Type 1 promoter of psbA2 gene in Synechocystis sp. PCC 6803

BBa_M11402
BBa_M11402 Version 1 (Component)
5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803

BBa_K639004
BBa_K639004 Version 1 (Component)
rrnB P1-LacI-pLac-mCherry plausible stress sensor

BBa_K1444010
BBa_K1444010 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - C1-434

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.