BBa_K390031
BBa_K390031 Version 1 (Component)
sigA promoter, 5' UTR and native RBS, and GFP

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP

BBa_K086008
BBa_K086008 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086006
BBa_K086006 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086005
BBa_K086005 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086009
BBa_K086009 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP

BBa_K654051
BBa_K654051 Version 1 (Component)
Promoter Tester 6803 Native RBS sigA Reference Dual Luciferase Assay

BBa_K654055
BBa_K654055 Version 1 (Component)
Promoter Tester 6803 Consensus RBS sigA Reference Dual Luciferase Assay

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086012
BBa_K086012 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086007
BBa_K086007 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP reporter

BBa_K086013
BBa_K086013 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086004
BBa_K086004 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP promoter

BBa_K086014
BBa_K086014 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086016
BBa_K086016 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086011
BBa_K086011 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086010
BBa_K086010 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086015
BBa_K086015 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K2116068
BBa_K2116068 Version 1 (Component)
AND gate regulated by norR and esaR (internal esabox, overlapping with sigma 54 binding site, before

BBa_K1994060
BBa_K1994060 Version 1 (Component)
Multiple dCas9 binding sites with weak sigma 54 promoter upstream of GFP + dCas9 expression cassette

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_M11410
BBa_M11410 Version 1 (Component)
Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

BBa_K1633005
BBa_K1633005 Version 1 (Component)
MOR siRNA-3 (siRNA for mouse Mu opioid receptor)

BBa_K1633004
BBa_K1633004 Version 1 (Component)
MOR siRNA-2 (siRNA for mouse Mu opioid receptor)

BBa_K1633003
BBa_K1633003 Version 1 (Component)
MOR siRNA-1 (siRNA for mouse Mu opioid receptor)

BBa_K1961007
BBa_K1961007 Version 1 (Component)
CYP1A2, an enzyme of cytochrome P450 protein family that metabolizes toxin in liver

BBa_K371054
BBa_K371054 Version 1 (Component)
MPF(meta-prefix)+[GFP+10*GS+A] fusion protein+MSF(meta-suffix))

BBa_K774102
BBa_K774102 Version 1 (Component)
Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide usin

BBa_K187005
BBa_K187005 Version 1 (Component)
Sigma 70 25% promoter in pAB, BioBytes plasmid

BBa_K187007
BBa_K187007 Version 1 (Component)
Sigma 70 75% promoter in pAB, Biobytes plasmid

BBa_K187014
BBa_K187014 Version 1 (Component)
Sigma 70 100% promoter in pBA, Biobytes plasimd

BBa_K187006
BBa_K187006 Version 1 (Component)
Sigma 70 50% promoter in pAB, Biobytes plasmid

BBa_K187003
BBa_K187003 Version 1 (Component)
Sigma 70 1% promoter in pAB BioBytes plasmid

BBa_K187004
BBa_K187004 Version 1 (Component)
Sigma 70 10% promoter in pAB, Biobytes plasmid

BBa_M11408
BBa_M11408 Version 1 (Component)
Type 1 promoter of sigA gene in Synechocystis sp. PCC 6803

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.