BBa_K1320000
BBa_K1320000 Version 1 (Component)
Cd sensitive promoter with Ogr activator, PO promoter, and GFP

BBa_K327015
BBa_K327015 Version 1 (Component)
Lux activated, C1lam repressed switch

BBa_I741016
BBa_I741016 Version 1 (Component)
Reverse Total XylR Transcriptional Regulator Left Facing (I741011 reverse complement

BBa_K1968014
BBa_K1968014 Version 1 (Component)
Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae

BBa_J58014
BBa_J58014 Version 1 (Component)
Promoter activated by OmpR-P with the reporter GFP

BBa_J58015
BBa_J58015 Version 1 (Component)
Mutated promoter activated by OmpR-P with the reporter GFP

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.