AraC_TEV-F
BBa_K627010 Version 1 (Component)
Fusion of AraC induction system and TEV protease 3

BBa_K385005
BBa_K385005 Version 1 (Component)
B-box sequence encoding a regulatory mRNA stem loop

Prom/RBS
BBa_K262000 Version 1 (Component)
BBa_R0011 & BBa_B0034, IPTG-inducible promoter with Elowitz RBS.

BBa_K2047012
BBa_K2047012 Version 1 (Component)
Stem loop with free energy of -44.9 measured by Mfold

BBa_K2047011
BBa_K2047011 Version 1 (Component)
Stem-loop with free energy of -51.4 kcal/mol, measured by Mfold

BBa_K2047010
BBa_K2047010 Version 1 (Component)
Stem loop with free energy of -38.8 kcal/mol measured by Mfold

BBa_K2047013
BBa_K2047013 Version 1 (Component)
Stem-loop with free energy of -10.0 kcal/mol measured by Mfold

BBa_K2047002
BBa_K2047002 Version 1 (Component)
Stem-loop with free energy of -14.9 kcal/mol measured by Mfold

BBa_K2047009
BBa_K2047009 Version 1 (Component)
Stem loop with free energy of -25.6 kcal/mol measured by Mfold

AraC RBS
BBa_K1392935 Version 1 (Component)
AraC-Pbad - Arabinose inducible regulatory promoter/repressor unit+ RBS

BBa_K1968009
BBa_K1968009 Version 1 (Component)
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger

BBa_J329040
BBa_J329040 Version 1 (Component)
luxIR QS System with B0034 RBSs and LVA-tagged GFP

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

pSBBs0K
BBa_K823026 Version 1 (Component)
pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_M36561
BBa_M36561 Version 1 (Component)
This terminator is a general terminator of transcription. It forms a stem loop which stops transcrip

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.