BBa_K957005
BBa_K957005 Version 1 (Component)
Constitutive expression of nah Operon for degradation of naphthalene to salicylate

Ag43 RCF10
BBa_K1352000 Version 1 (Component)
Ag43 with PstI sites removed under the control of pBad/araC promoter

S-protein
BBa_M0029 Version 1 (Component)
S-protein affinity tag

EYFP
BBa_E0036 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ECFP
BBa_E0026 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

cheB
BBa_C0024 Version 1 (Component)
CheB chemotaxis coding sequence (protein glutamate methylesterase)

BBa_K797008
BBa_K797008 Version 1 (Component)
Plasmid include T7-RBS-R9 region

BBa_K929106
BBa_K929106 Version 1 (Component)
B-cell transmembrane region with mCherry

LMR
BBa_K175031 Version 1 (Component)
Lock for medium RBS (B0032) from the lock/key library TUD09

BBa_T1002
BBa_T1002 Version 1 (Component)
Testing the categories

BBa_I0460
BBa_I0460 Version 1 (Component)
aiiA Device (B0034.C0060.B0015)

BBa_K1523103
BBa_K1523103 Version 1 (Component)
The translational unit of yieF

BBa_K1351017
BBa_K1351017 Version 1 (Component)
SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>

BBa_K1394002
BBa_K1394002 Version 1 (Component)
MAP star peptide

BBa_I711001
BBa_I711001 Version 1 (Component)
generates the tyrosinase for melanin biosynthesis

BBa_M34014
BBa_M34014 Version 1 (Component)
Nucleotide sequence of a cDNA clone of saxiphilin from the liver of Rana Catesbeiana

BBa_I714000
BBa_I714000 Version 1 (Component)
R388 oriT ( transfer origin of E.coli IncW group R388 plasmid)

LacI_I12
BBa_K091122 Version 1 (Component)
LacI_I12 protein

BBa_I737004
BBa_I737004 Version 1 (Component)
AraC, arabinose activated, self sustaining GFP reporter.

BBa_K238020
BBa_K238020 Version 1 (Component)
sam genes with promoter

BBa_I756001
BBa_I756001 Version 1 (Component)
Eukaryotic Protein generator for DOCK11

BBa_K1919000
BBa_K1919000 Version 1 (Component)
The coding sequence of antimicrobial peptide CecropinXJ

BBa_J58109
BBa_J58109 Version 1 (Component)
Trg-EnvZ coding region with terminator

BBa_K176006
BBa_K176006 Version 1 (Component)
PoPS->RBS+tetR-LVA+T

BBa_K786002
BBa_K786002 Version 1 (Component)
Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light

BBa_M36114
BBa_M36114 Version 1 (Component)
Sylvia's Glycerol Stock of the Mutated Cell

BBa_M36112
BBa_M36112 Version 1 (Component)
Matt's Glycerol Stock of the mutant E.Coli

BBa_K299805
BBa_K299805 Version 1 (Component)
J23100+scar

BBa_K1897032
BBa_K1897032 Version 1 (Component)
B0032 with flanking Scar sites

"The Blue
BBa_K498000 Version 1 (Component)
T9002 - LacZ

BBa_K1431503
BBa_K1431503 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431501
BBa_K1431501 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431502
BBa_K1431502 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1051801
BBa_K1051801 Version 1 (Component)
Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib

BBa_K587005
BBa_K587005 Version 1 (Component)
iTast -> inhibits the action of tast

BBa_K888000
BBa_K888000 Version 1 (Component)
L2 protein of adhesion to silica

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.