BBa_K209000
BBa_K209000 Version 1 (Component)
cAR1 (Dictyostelium discoideum), no STOP

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086012
BBa_K086012 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086007
BBa_K086007 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP reporter

BBa_K086013
BBa_K086013 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086004
BBa_K086004 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP promoter

BBa_K086014
BBa_K086014 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086016
BBa_K086016 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K086011
BBa_K086011 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086010
BBa_K086010 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32 followed by YFP reporter

BBa_K086015
BBa_K086015 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter

BBa_K2116068
BBa_K2116068 Version 1 (Component)
AND gate regulated by norR and esaR (internal esabox, overlapping with sigma 54 binding site, before

BBa_K1994060
BBa_K1994060 Version 1 (Component)
Multiple dCas9 binding sites with weak sigma 54 promoter upstream of GFP + dCas9 expression cassette

BBa_K101015
BBa_K101015 Version 1 (Component)
Standard Promoter with RBS, GFP and terminator

BBa_M11410
BBa_M11410 Version 1 (Component)
Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse

BBa_K792000
BBa_K792000 Version 1 (Component)
Yeast exportable His-rich peptide w/enhanced import (nonStd)

BBa_K1053201
BBa_K1053201 Version 1 (Component)
Pconst.-SRⅡ-HtrⅡ-EnvZ-Pompc-HHR*-GFP-DT

BBa_J36852
BBa_J36852 Version 1 (Component)
Streptavidin, single-chain dimer (no start codon)

BBa_K187005
BBa_K187005 Version 1 (Component)
Sigma 70 25% promoter in pAB, BioBytes plasmid

BBa_K187007
BBa_K187007 Version 1 (Component)
Sigma 70 75% promoter in pAB, Biobytes plasmid

BBa_K187014
BBa_K187014 Version 1 (Component)
Sigma 70 100% promoter in pBA, Biobytes plasimd

BBa_K187006
BBa_K187006 Version 1 (Component)
Sigma 70 50% promoter in pAB, Biobytes plasmid

BBa_K187003
BBa_K187003 Version 1 (Component)
Sigma 70 1% promoter in pAB BioBytes plasmid

BBa_K187004
BBa_K187004 Version 1 (Component)
Sigma 70 10% promoter in pAB, Biobytes plasmid

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.