BBa_K896599BBa_K896599 Version 1 (Component)Strophurus rankini voucher AMS R140490 NADH dehydrogenase subunit 4 (ND4) gene, partial cds; tRNA-Hi
iGEM 2019 PlatesiGEM_2019_Plates Version 1 (Collection)384-well plates of dried DNA distributed by iGEM in Spring 2019
iGEM 2018 PlatesiGEM_2018_Plates Version 1 (Collection)384-well plates of dried DNA distributed by iGEM in Spring 2018
BBa_K1124100BBa_K1124100 Version 1 (Component)clock device (plux/tet) (+luxI, +tetR, +aiiA, ++sRNA, +amilGFP, +UV reset device(E234K, -LVA)
BBa_K880001BBa_K880001 Version 1 (Component)Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K
BBa_K323151BBa_K323151 Version 1 (Component)DNA program 123456
BBa_K323152BBa_K323152 Version 1 (Component)DNA Program 12346
BBa_K801032BBa_K801032 Version 1 (Component)GAL4 DNA binding domain
BBa_K105101BBa_K105101 Version 1 (Component)Gal4 - DNA binding domain
BBa_K105100BBa_K105100 Version 1 (Component)LacI - DNA binding domain
BBa_K887011BBa_K887011 Version 1 (Component)DNA program : specific DNA sequence which can be recognized by zinc fingers
BBa_K199091BBa_K199091 Version 1 (Component)pLac-CUACC Suppressor tRNA
BBa_K779120BBa_K779120 Version 1 (Component)RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock
BBa_J119088BBa_J119088 Version 1 (Component)pT7-RBS-GFP-tRNA (K191)
BBa_J119087BBa_J119087 Version 1 (Component)pT7-RBS-GFP-tRNA (K191)
CmsRNABBa_K1960102 Version 1 (Component)sRNA, which can silence chloramphenicol resistance
BBa_K199170BBa_K199170 Version 1 (Component)T7 Promoter RBS RFP pLac CCACC (tRNA)
BBa_K199169BBa_K199169 Version 1 (Component)T7 Promoter RBS RFP pLac CUAGC (tRNA)
BBa_K187254BBa_K187254 Version 1 (Component)folD, ORF, forward primer
BBa_K1441013BBa_K1441013 Version 1 (Component)DNA ligase from Escherichia coli with His-tag INSERT
BBa_K809710BBa_K809710 Version 1 (Component)GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator
BBa_K779122BBa_K779122 Version 1 (Component)Short DNA reporter bottom strand (with ROX fluorophore) MammoBlock
BBa_K779121BBa_K779121 Version 1 (Component)Short DNA reporter top strand (with RQ quencher) MammoBlock
PchiP-lacZBBa_K564002 Version 1 (Component)Chitoporin fused with lacZ - target for sRNA based regulation
BBa_K199070BBa_K199070 Version 1 (Component)I13453:K199016 Pbad promotor with the suppressor tRNA of CUAGC
BBa_K1942000BBa_K1942000 Version 1 (Component)A shRNA corresponding DNA sequence for KRAS which could silence the gene
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
BBa_K199071BBa_K199071 Version 1 (Component)I13453:K199014: Pbad promomtor with the suppressor tRNA of the codon AGGAC
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.