ZF2
BBa_K655001 Version 1 (Component)
Zinc Finger Protein (ZF2) (binding site: TAGGATGGG)

ZF5
BBa_K655004 Version 1 (Component)
Zinc Finger Protein (ZF5) (binding site: GTGGAGGCT)

BBa_K371024
BBa_K371024 Version 1 (Component)
10*GS linker(for RFC 53 protein fusion)

BBa_K323033
BBa_K323033 Version 1 (Component)
ATG cYFP link Jazz link His stop T7 terminator

BBa_K323045
BBa_K323045 Version 1 (Component)
ATG cYFP link PBSII link His stop T7 terminator

BBa_K323024
BBa_K323024 Version 1 (Component)
ATG cCFP link Jazz link His stop T7 terminator

BBa_K133082
BBa_K133082 Version 1 (Component)
CMV-SS-HA-UreB-gyrEC-2xlinker

BBa_K1780000
BBa_K1780000 Version 1 (Component)
variable link oscillator

BBa_K624064
BBa_K624064 Version 1 (Component)
nested_Inv R (inter-strain nested primer)

BBa_K624063
BBa_K624063 Version 1 (Component)
nested_Inv F (inter-strain nested primer)

BBa_K624065
BBa_K624065 Version 1 (Component)
nested_LLO F (inter-strain nested primer)

BBa_K624066
BBa_K624066 Version 1 (Component)
nested_LLO R (inter-strain nested primer)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2039002
BBa_K2039002 Version 1 (Component)
Dimerization system FRB/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)

BBa_K1317003
BBa_K1317003 Version 1 (Component)
CDS for Elastin like polypeptide (ELP)

BBa_K1780001
BBa_K1780001 Version 1 (Component)
variable link oscillator

BBa_K1051102
BBa_K1051102 Version 1 (Component)
targeting peptide to mitochondria inner membrane

BBa_K1051100
BBa_K1051100 Version 1 (Component)
targeting peptide to mitochondria inner membrane

BBa_K133084
BBa_K133084 Version 1 (Component)
CMV-SS-gyrHP-2xlinker-(TMTIR)TLR9-Histop

BBa_K887060
BBa_K887060 Version 1 (Component)
B0034(RBS)+HivC(zinc finger)+ilvD+37

BBa_K133085
BBa_K133085 Version 1 (Component)
CMV-SS-gyrEC-2xlinker-(TMTIR)TLR9-Histop

BBa_K323044
BBa_K323044 Version 1 (Component)
Jazz link nCFP stop

BBa_K323050
BBa_K323050 Version 1 (Component)
Gli1 link nCFP stop

BBa_K323028
BBa_K323028 Version 1 (Component)
Jazz link nYFP stop

BBa_K323051
BBa_K323051 Version 1 (Component)
ATG cCFP link Zif268

BBa_K323031
BBa_K323031 Version 1 (Component)
HIVC link nCFP stop

BBa_K323056
BBa_K323056 Version 1 (Component)
PBSII link nYFP stop

BBa_K323148
BBa_K323148 Version 1 (Component)
CMV ATG cYFP link Zif268 link His stop term

BBa_K929200
BBa_K929200 Version 1 (Component)
N-terminal sortase motif linked to AAV2-VP2 with Myc-tag, kozak and CMV

BBa_K1480001
BBa_K1480001 Version 1 (Component)
Transcription Activator-Like Effector Nuclease (TALEN)

BBa_K323076
BBa_K323076 Version 1 (Component)
ATG cYFP link Blues link His stop T7 terminator

BBa_K1149009
BBa_K1149009 Version 1 (Component)
Cutinase-Like Enzyme

BBa_K1833006
BBa_K1833006 Version 1 (Component)
lac promoter -> Zinc finger 225 binding domain fused to Gal11P

BBa_J23076
BBa_J23076 Version 1 (Component)
pSB3C6-like RFP reporter

BBa_I717014
BBa_I717014 Version 1 (Component)
TF to FP link

BBa_K323006
BBa_K323006 Version 1 (Component)
ATG cCFP link Gli1

BBa_K1189031
BBa_K1189031 Version 1 (Component)
TALE-A linked to beta-lactamase with a his tag under a lacI promoter

BBa_M36087
BBa_M36087 Version 1 (Component)
Winter Flounder Antifreeze Protein Actuator without Secretion Tag

BBa_M36085
BBa_M36085 Version 1 (Component)
Winter Flounder Antifreeze Protein Actuator With Secretion Tag

BBa_K606063
BBa_K606063 Version 1 (Component)
T7 amber expression monitored by a linear system

BBa_K1051101
BBa_K1051101 Version 1 (Component)
Cox4 targeting peptide to mitochondria inner membrane

CymA
BBa_K1748004 Version 1 (Component)
Inner-membrane tetraheme c-type cytochrome CymA

T
BBa_K896949 Version 1 (Component)
promoter and MpV17 mitochondrial inner membrane protein

SAMT
BBa_J45001 Version 1 (Component)
SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter

BBa_K2039007
BBa_K2039007 Version 1 (Component)
Dimerization system FRB/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9)

BBa_K125550
BBa_K125550 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_K1597002
BBa_K1597002 Version 1 (Component)
tasA, amyloid-like fibers

BBa_K2122151
BBa_K2122151 Version 1 (Component)
Sub unit B Shiga-like toxin

BBa_K1974021
BBa_K1974021 Version 1 (Component)
T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974023
BBa_K1974023 Version 1 (Component)
T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag