BBa_K1253114
BBa_K1253114 Version 1 (Component)
This part is used to test for TetR half-life.

BBa_I759006
BBa_I759006 Version 1 (Component)
pTet-spacer-GFP

BBa_K631009
BBa_K631009 Version 1 (Component)
OX1R (GPCR; for use in C. elegans)

TetR-4C
BBa_I12212 Version 1 (Component)
TetR - TetR-4C heterodimer promoter (negative)

BBa_I13607
BBa_I13607 Version 1 (Component)
Lac YFP Tet CFP (Ly+Tc+)

BBa_J06201
BBa_J06201 Version 1 (Component)
Tet repressible rhI generator (LVA+)

BBa_J06002
BBa_J06002 Version 1 (Component)
Tet repressible luxI generator ( LVA- )

BBa_J06101
BBa_J06101 Version 1 (Component)
Tet repressible lasI generator ( LVA+ )

BBa_J06102
BBa_J06102 Version 1 (Component)
Tet repressible lasI generator ( LVA- )

BBa_M1161
BBa_M1161 Version 1 (Component)
Test

BBa_J44008
BBa_J44008 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

BBa_J44006
BBa_J44006 Version 1 (Component)
pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

BBa_K142019
BBa_K142019 Version 1 (Component)
tet-controlled lacI IS mutant (T276F) generator

BBa_K142016
BBa_K142016 Version 1 (Component)
tet-controlled lacI IS mutant (R197A) generator

BBa_K142017
BBa_K142017 Version 1 (Component)
tet-controlled lacI IS mutant (R197F) generator

BBa_K142018
BBa_K142018 Version 1 (Component)
tet-controlled lacI IS mutant (T276A) generator

BBa_K249010
BBa_K249010 Version 1 (Component)
C-terminal YFP --> Tet promoter

BBa_K208031
BBa_K208031 Version 1 (Component)
Tet Promoter/RBS/PhaP/Terminator

BBa_K1124202
BBa_K1124202 Version 1 (Component)
pLux/tet-mCherry-luxI-aiiA

BBa_K249012
BBa_K249012 Version 1 (Component)
N-terminal YFP --> Tet promoter

BBa_I13115
BBa_I13115 Version 1 (Component)
High output control (tet QPI)

BBa_K727003
BBa_K727003 Version 1 (Component)
Trimethylamine Methyltransferase (TMT)

BBa_K1875011
BBa_K1875011 Version 1 (Component)
This part produces a guide RNA that pairs with an operator.

BBa_K1875012
BBa_K1875012 Version 1 (Component)
This part produces a guide RNA that pairs with an operator.

BBa_K1385000
BBa_K1385000 Version 1 (Component)
CPCG2 promoter -> TetR

BBa_K1510104
BBa_K1510104 Version 1 (Component)
terminator in S.mutans

BBa_K1370000
BBa_K1370000 Version 1 (Component)
hmp in pSB1C3

BBa_J44007
BBa_J44007 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

BBa_J44005
BBa_J44005 Version 1 (Component)
pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

BBa_K1400003
BBa_K1400003 Version 1 (Component)
pTre(4) Single input tet responsive promoter

BBa_K624046
BBa_K624046 Version 1 (Component)
Pmsp1 (tetO)

BBa_K1583112
BBa_K1583112 Version 1 (Component)
pRha + CsgA & GFP in same operon

BBa_K879811
BBa_K879811 Version 1 (Component)
Red with Tet inducible promoter

BBa_K879812
BBa_K879812 Version 1 (Component)
Blue with Tet inducible promoter

BBa_S03808
BBa_S03808 Version 1 (Component)
cis3-repressed, tet-regulated Q

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SET
BBa_K1433011 Version 1 (Component)
Terminator-RFP-RBS-attB-P-attP-RBS-GFP-Terminator

BBa_J24687
BBa_J24687 Version 1 (Component)
8 M RBS - TetR

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

BBa_J70575
BBa_J70575 Version 1 (Component)
{15,18;13,10} BsaXI BioScaffold v 5.0, no BsaXI, V169I in E0040

BBa_J24686
BBa_J24686 Version 1 (Component)
7. W RBS - TetR

BBa_K1222005
BBa_K1222005 Version 1 (Component)
pGFP(T7 promoter+lac operator GFP antisense 2+T7 terminator)

ROP+P
BBa_K809403 Version 1 (Component)
ROP+Promoter in mitochondria

P+ROP
BBa_K809402 Version 1 (Component)
Promoter+ROP in mitochondria

BBa_K909011
BBa_K909011 Version 1 (Component)
Dual input promoter: PL promoter with two operator sites TetO1 and LacO1

BBa_K132009
BBa_K132009 Version 1 (Component)
Ptet+cfp-LVA+Ptet

BBa_I718015
BBa_I718015 Version 1 (Component)
ter-lox66-rbs-dapA (B. Subtilis)