BBa_K313006
BBa_K313006 Version 1 (Component)
MS2 phage cDNA (XbaI site is removed)

BBa_K2122000
BBa_K2122000 Version 1 (Component)
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter

SPtom22
BBa_K809206 Version 1 (Component)
signal peptide of Tom22

BBa_K2055016
BBa_K2055016 Version 1 (Component)
Part designed for verification and characterization of the FUR/LacI inverter

ihfA
BBa_K112218 Version 1 (Component)
{rbs.ihfA!} The ihf alpha gene with native rbs and stop codon, BBb format

ihfB
BBa_K112224 Version 1 (Component)
{rbs.ihfB!} The ihf beta gene with native rbs and stop codon, BBb format

BBa_K1826001
BBa_K1826001 Version 1 (Component)
The part encodes the bacteriocin bactofencinA

T
BBa_G0012 Version 1 (Component)
The nucleotide T

BBa_K624012
BBa_K624012 Version 1 (Component)
RBS from Pmsp3

BBa_K112216
BBa_K112216 Version 1 (Component)
{< ihfA >} The ihf alpha gene without start and stop codons, BBb format

BBa_K136005
BBa_K136005 Version 1 (Component)
FlhDC gene from

BBa_K1377000
BBa_K1377000 Version 1 (Component)
promoter of pili A gene cluster + spaA front

EHEC_TALE1
BBa_K1189032 Version 1 (Component)
EHEC TALE1 (binds to 19bp of stx2 gene)

myc-gene I
BBa_K627007 Version 1 (Component)
Fusion of c-myc-tag and gene III

BBa_K2139018
BBa_K2139018 Version 1 (Component)
Coding Sequence of G12

Cv2935
BBa_K1293023 Version 1 (Component)
Functional chitinase gene (Cv2935) of Chromobacterium violaceum

BBa_K850001
BBa_K850001 Version 1 (Component)
NAG1 gene from candida albicans

BBa_C100010
BBa_C100010 Version 1 (Component)
ORF of LIS odorant enzyme

BBa_C100009
BBa_C100009 Version 1 (Component)
ORF of LMS odorant enzyme

BBa_K1641013
BBa_K1641013 Version 1 (Component)
SloxM1, modified recognition site of recombinase Scre

BBa_K177010
BBa_K177010 Version 1 (Component)
invasine locus from yersinia

BBa_K346089
BBa_K346089 Version 1 (Component)
T7 promoter(BBa_I719005)+MerT+MerP+MerC(the facilitation module of mercury absorption)

BBa_K1510931
BBa_K1510931 Version 1 (Component)
Sequence integrated the luxR gene and pluxR promoter together.

BBa_K660614
BBa_K660614 Version 1 (Component)
T4 Endolysin + TT

BBa_C100006
BBa_C100006 Version 1 (Component)
ORF for the BEAT odorant enzyme

BBa_K310006
BBa_K310006 Version 1 (Component)
ArsR under the control of J23100, includes RBS B0034 and terminator B0015

BBa_B0022
BBa_B0022 Version 1 (Component)
TE from coliphageT7, reversed

BBa_M36028
BBa_M36028 Version 1 (Component)
phiX174 gene D - mutated rbs and start codon of E, and rbs of J

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_E0029
BBa_E0029 Version 1 (Component)
lacZalpha fragment

NpuDnaE(N)
BBa_K1362400 Version 1 (Component)
NpuDnaE N-Intein cloning piece

BBa_K1051704
BBa_K1051704 Version 1 (Component)
Mer2 Intron

BBa_K1172401
BBa_K1172401 Version 1 (Component)
mtrCAB - gene cluster from S. oneidensis for cytochrome expression

BBa_J70524
BBa_J70524 Version 1 (Component)
hard codes sequence of J70514 into a part

BBa_J70523
BBa_J70523 Version 1 (Component)
hard codes sequence of J70518 into a part

BBa_J70521
BBa_J70521 Version 1 (Component)
hard codes sequence of J70516 into a part

BBa_J70522
BBa_J70522 Version 1 (Component)
hard codes sequence of J70517 into a part

BBa_J70520
BBa_J70520 Version 1 (Component)
hard codes sequence of J70515 into a part

BBa_K1639008
BBa_K1639008 Version 1 (Component)
Tev Protease, single S219V mutation in the internal cleavage site

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

RBS
BBa_K1642004 Version 1 (Component)
SD sequence of cpcB

BBa_K798005
BBa_K798005 Version 1 (Component)
Recognition sequence of ribozyme sequence

tuned pArs
BBa_K935002 Version 1 (Component)
E. coli ArsR under the control of pArs coupled with pArs + RFP

BBa_K112222
BBa_K112222 Version 1 (Component)
{< ihfB >} The ihf beta gene without start or stop codons, BBb format

BBa_J11011
BBa_J11011 Version 1 (Component)
variant of J11005 uncoupled from CI(ts) production

BBa_K1610402
BBa_K1610402 Version 1 (Component)
T4 Endo + Term

BBa_K1935016
BBa_K1935016 Version 1 (Component)
White Collar 2 under the control of pU6 promoter in C.elegans (pU6::WC2)

BBa_K1465209
BBa_K1465209 Version 1 (Component)
Carbonic anhydrase (csoS3), shell-proteins (csoS14) and pore protein (csoS1D) of the carboxysome

BBa_K1935014
BBa_K1935014 Version 1 (Component)
White Collar 1 under the control of pU6 promoter in C.elegans (pU6::WC1)

BBa_K2082232
BBa_K2082232 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with Gal11P:cI(434)