BBa_J06650
BBa_J06650 Version 1 (Component)
Test of BBa_R0051

BBa_K594015
BBa_K594015 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces lasI and EYFP

RBS-pcyA
BBa_K193402 Version 1 (Component)
RBS is located upstream of pcyA

T18 domain
BBa_K1638004 Version 1 (Component)
T18 domain of CyaA from Bordetella pertussis

CBPa-BpA
BBa_K2053001 Version 1 (Component)
Fusion cellulose-binding domain of CBP-B domain of protein A

BBa_K1937007
BBa_K1937007 Version 1 (Component)
AF-A : operon of antifungal peptides

BBa_K1365111
BBa_K1365111 Version 1 (Component)
dspB with signal sequence of USP45

BBa_K374002
BBa_K374002 Version 1 (Component)
the Gifsy-2 phage lysogenic repressor gtgR

BBa_K374000
BBa_K374000 Version 1 (Component)
the Gifsy-1 phage lysogenic repressor gogR

BBa_K1660005
BBa_K1660005 Version 1 (Component)
RFP controlled by the PompR promoter

YFP_SPtom4
BBa_K809316 Version 1 (Component)
YFP + signal peptide of Tom40

BBa_J23080
BBa_J23080 Version 1 (Component)
b0034 derived RBS to match spacing of J01122

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_K1001758
BBa_K1001758 Version 1 (Component)
AraC coding upstream of Lux promoter upstream of GFP

BBa_I11013
BBa_I11013 Version 1 (Component)
Modification of biobricks part BBa_B0015

Strong+Imm
BBa_K320006 Version 1 (Component)
Constitutive expression of Immunotoxin

BBa_K1058003
BBa_K1058003 Version 1 (Component)
Promoter of penicillinase blaZ as well as promoter of blaR1

BBa_K1583013
BBa_K1583013 Version 1 (Component)
CsgA missing the start codon for fusion protein

BBa_K189061
BBa_K189061 Version 1 (Component)
Gp16 of Bacteriophage Mu

BBa_K835002
BBa_K835002 Version 1 (Component)
cytoplasmic region of tar

BBa_K189065
BBa_K189065 Version 1 (Component)
E18 of Bacteriophage Mu

BBa_K189068
BBa_K189068 Version 1 (Component)
C of Bacteriophage Mu

BBa_J100020
BBa_J100020 Version 1 (Component)
Second Half of AspC

BBa_K189052
BBa_K189052 Version 1 (Component)
GP7 of Bacteriophage Mu

BBa_K189050
BBa_K189050 Version 1 (Component)
Kil of Bacteriophage Mu

BBa_M30050
BBa_M30050 Version 1 (Component)
Refactoring of M13K07 genome

BBa_K189075
BBa_K189075 Version 1 (Component)
Gp28 of Bacteriophage Mu

BBa_K189056
BBa_K189056 Version 1 (Component)
Gp11 of Bacteriophage Mu

BBa_K189062
BBa_K189062 Version 1 (Component)
Gp16 of Bacteriophage Mu

BBa_K189057
BBa_K189057 Version 1 (Component)
Gp12 of Bacteriophage Mu

BBa_J61052
BBa_J61052 Version 1 (Component)
[neuS1] of K1 ORF

BBa_K189051
BBa_K189051 Version 1 (Component)
Gp6 of Bacteriophage Mu

BBa_K189070
BBa_K189070 Version 1 (Component)
Gp23 of Bacteriophage Mu

BBa_K189066
BBa_K189066 Version 1 (Component)
E19 of Bacteriophage Mu

BBa_I714002
BBa_I714002 Version 1 (Component)
traI of F plasmid

BBa_K189071
BBa_K189071 Version 1 (Component)
Gp24 of Bacteriophage Mu

BBa_K189048
BBa_K189048 Version 1 (Component)
A of Bacteriophage Mu

BBa_K189058
BBa_K189058 Version 1 (Component)
Gp13 of Bacteriophage Mu

BBa_K189054
BBa_K189054 Version 1 (Component)
GP9 of Bacteriophage Mu

BBa_K189076
BBa_K189076 Version 1 (Component)
Gp29 of Bacteriophage Mu

Strong+P25
BBa_K320007 Version 1 (Component)
Constitutive expression of P25

BBa_K189074
BBa_K189074 Version 1 (Component)
Gp27 of Bacteriophage Mu

BBa_K1650010
BBa_K1650010 Version 1 (Component)
Biosynthesis of beta-carotene

BBa_K189064
BBa_K189064 Version 1 (Component)
Mor of Bacteriophage Mu

BBa_K769002
BBa_K769002 Version 1 (Component)
Kinase domain of EnvZ

BBa_J100022
BBa_J100022 Version 1 (Component)
Second Half of PyrE

BBa_K189053
BBa_K189053 Version 1 (Component)
GP8 of Bacteriophage Mu

BBa_J72161
BBa_J72161 Version 1 (Component)
ColE2 origin of replication

BBa_K189073
BBa_K189073 Version 1 (Component)
Gp26 of Bacteriophage Mu