BBa_K195403BBa_K195403 Version 1 (Component)This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv
BBa_K1947023BBa_K1947023 Version 1 (Component)This part serves as a catch system expressed in <i>E. coli.
BBa_K896906BBa_K896906 Version 1 (Component)this is a hormone produced mainly in guts used for controlling the appetite
GG100BBa_K2145125 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
GG98BBa_K2145123 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
BBa_K1367003BBa_K1367003 Version 1 (Component)This plasmid containing siRNA for LAR gene in Camelia sinensis, used to knockdown gene LAR.
BBa_K1932007BBa_K1932007 Version 1 (Component)This device is constructed for the expression of TAT-apoptin fused with tmp1.
BBa_M36916BBa_M36916 Version 1 (Component)This series of parts codes for the expression of the benzylalcohol acetyltransferase enzyme
BBa_K195404BBa_K195404 Version 1 (Component)This part is composed of pTetR,RBS,Lpp-OmpA-linker,F10scFv and terminator
BBa_M36561BBa_M36561 Version 1 (Component)This terminator is a general terminator of transcription. It forms a stem loop which stops transcrip
BBa_J70652BBa_J70652 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
BBa_K362007BBa_K362007 Version 1 (Component)This composite part has the promoter ,ahpC-promoter controlled by OxyR protein, and GFP - T-T
BBa_K1114302BBa_K1114302 Version 1 (Component)This is <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_B001
BBa_J70653BBa_J70653 Version 1 (Component)This tests adding an internal strep tag linker to a protein using J70590 as a te
[OriTRP4]+BBa_K1439001 Version 1 (Component)This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.
BBa_K1456014BBa_K1456014 Version 1 (Component)Oxygen dependent degradation(ODD) domain from HIF-1a reverse primer with restriction site
BBa_K1456013BBa_K1456013 Version 1 (Component)Oxygen dependent degradation(ODD) domain from HIF-1a forward primer with restriction site
BBa_K1655001BBa_K1655001 Version 1 (Component)This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.
Adapter BiBBa_K1807015 Version 1 (Component)This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of
Bm3R1BBa_K1401000 Version 1 (Component)TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')
BBa_K188006BBa_K188006 Version 1 (Component)Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be
BBa_T1010BBa_T1010 Version 1 (Component)Kill this test
BBa_K737000BBa_K737000 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K1942001BBa_K1942001 Version 1 (Component)This part is a short RNA sequence designed for KRAS gene silencing. It is used for down-regulating K
BBa_K987001BBa_K987001 Version 1 (Component)This is a composite part which has the function to invert the temperature activation by the part: BB
BBa_K987000BBa_K987000 Version 1 (Component)This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p
CMV + GFPBBa_K1852000 Version 1 (Component)GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report
BBa_J580110BBa_J580110 Version 1 (Component)This is my first part
BBa_K783040BBa_K783040 Version 1 (Component)This is a MoClo converted version of BBa_J23110
BBa_K783034BBa_K783034 Version 1 (Component)This is a MoClo converted version of BBa_J23114
BBa_K896986BBa_K896986 Version 1 (Component)this is a gene about a T cell receptor
BBa_K294205BBa_K294205 Version 1 (Component)This is a coding sequence of heat shock protein from E.coli
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
BBa_K2150010BBa_K2150010 Version 1 (Component)This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included
BBa_K737001BBa_K737001 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.