BBa_K2066105
BBa_K2066105 Version 1 (Component)
Promoter characterization part - J23107 with RiboJ

BBa_K2066074
BBa_K2066074 Version 1 (Component)
Promoter characterization part: J23116 with RiboJ

BBa_K2066061
BBa_K2066061 Version 1 (Component)
Promoter characterization part: J23101 with RiboJ

BBa_K2066065
BBa_K2066065 Version 1 (Component)
Promoter characterization part: J23105 with RiboJ

BBa_K2066047
BBa_K2066047 Version 1 (Component)
B0035 IPTG-Inducible RBS Measurement Part

BBa_K2066049
BBa_K2066049 Version 1 (Component)
B0033 IPTG-inducible RBS Measurement Part

BBa_K2066077
BBa_K2066077 Version 1 (Component)
Promoter characterization part - J23119 with RiboJ

BBa_K2066045
BBa_K2066045 Version 1 (Component)
B0064 IPTG-inducible RBS Measurement Part

BBa_K2066072
BBa_K2066072 Version 1 (Component)
Promoter characterization part: J23113 with RiboJ

BBa_K2066070
BBa_K2066070 Version 1 (Component)
Promoter characterization part: J23111 with RiboJ

SP-aprE
BBa_K1674000 Version 1 (Component)
Secretion signal peptide originated from the B.subtilis extracellular protease AprE

BBa_M1703
BBa_M1703 Version 1 (Component)
Inulosucrase and scrT sucrose transport protein composite part

BBa_K1758330
BBa_K1758330 Version 1 (Component)
Lead repressor under control of constitutive promoter and strong RBS

BBa_K1392972
BBa_K1392972 Version 1 (Component)
Composite part fot TetLVA TetPromoter and T4

BBa_K137115
BBa_K137115 Version 1 (Component)
Constitutively expresses cI, the lambda repressor.

Pmra
BBa_S03331 Version 1 (Component)
--Specify Parts List--

BBa_I10099
BBa_I10099 Version 1 (Component)
coding region of FimH protein of E.coli

BBa_M12100
BBa_M12100 Version 1 (Component)
PLambda : B0034 : RFP in BioScaffold Form, a (0,5;7,2] BioScaffold part

BBa_K2066035
BBa_K2066035 Version 1 (Component)
B0034 IPTG-inducible RBS Measurement Part

BBa_K2066036
BBa_K2066036 Version 1 (Component)
B0031 IPTG-inducible RBS Measurement Part

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_K1758105
BBa_K1758105 Version 1 (Component)
mRFP generator under control of T7 promoter

BBa_K1051801
BBa_K1051801 Version 1 (Component)
Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib

BBa_J22146
BBa_J22146 Version 1 (Component)
The Lac Y and mRFP gene under the RecA(SOS) promoter.

BBa_I746321
BBa_I746321 Version 1 (Component)
GFP under control of PO promoter

BBa_K1390014
BBa_K1390014 Version 1 (Component)
mmoC-His

BBa_K1390016
BBa_K1390016 Version 1 (Component)
mmoX-His

BBa_K1390015
BBa_K1390015 Version 1 (Component)
mmoD-His

BBa_K1390018
BBa_K1390018 Version 1 (Component)
mmoZ-His

BBa_K1390013
BBa_K1390013 Version 1 (Component)
mmoB-His

Fos
BBa_K243027 Version 1 (Component)
His-FOS

BBa_K1390017
BBa_K1390017 Version 1 (Component)
mmoY-His

BBa_K575005
BBa_K575005 Version 1 (Component)
Continuous expression of LasR receptor protein (with RBS, B0034)

BBa_K1669003
BBa_K1669003 Version 1 (Component)
CtfB + His-tag

BBa_K1669004
BBa_K1669004 Version 1 (Component)
BdhB + His-tag

BBa_K1669001
BBa_K1669001 Version 1 (Component)
CtfA + His-tag

BBa_K2066052
BBa_K2066052 Version 1 (Component)
J23107 without RiboJ Promoter Characterization Part

BBa_K077048
BBa_K077048 Version 1 (Component)
Testing part BBa_R0062, by GFP output.

BBa_K2066043
BBa_K2066043 Version 1 (Component)
J23115 without RiboJ Promoter Characterization Part

BBa_K2066041
BBa_K2066041 Version 1 (Component)
J23100 without RiboJ Promoter Characterization Part

BBa_K1447006
BBa_K1447006 Version 1 (Component)
HIS-TEV

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1509003
BBa_K1509003 Version 1 (Component)
CP25 is a highly active,constitutive promoter

BBa_K1149033
BBa_K1149033 Version 1 (Component)
pudA-his

Riboswitch
BBa_J04700 Version 1 (Component)
Part containing promoter, riboswitch mTCT8-4 theophylline aptamer (J04705), and RBS

BBa_K1159204
BBa_K1159204 Version 1 (Component)
Secretory SpyCatcher (SERK-SigP_SpyCatcher) in RFC[25] N-Part

BBa_K1159205
BBa_K1159205 Version 1 (Component)
Secretory SpyTag (SERK-SigP_SpyTag) in RFC[25] N-Part

BBa_K902082
BBa_K902082 Version 1 (Component)
TetR Promoter-moco riboswitch-GFP LacI Promoter-rbs

BBa_K1520000
BBa_K1520000 Version 1 (Component)
MlrD.The protein is involved in microcystin degradation.

BBa_K1055001
BBa_K1055001 Version 1 (Component)
LssmOrange is a fluorophor derived from dsRed