BBa_K187007BBa_K187007 Version 1 (Component)Sigma 70 75% promoter in pAB, Biobytes plasmid
BBa_K187014BBa_K187014 Version 1 (Component)Sigma 70 100% promoter in pBA, Biobytes plasimd
BBa_K187006BBa_K187006 Version 1 (Component)Sigma 70 50% promoter in pAB, Biobytes plasmid
BBa_K187003BBa_K187003 Version 1 (Component)Sigma 70 1% promoter in pAB BioBytes plasmid
BBa_K187004BBa_K187004 Version 1 (Component)Sigma 70 10% promoter in pAB, Biobytes plasmid
BBa_I733004BBa_I733004 Version 1 (Component)Produce LacZ alpha in response to AHL
BBa_K812133BBa_K812133 Version 1 (Component)sfGFP with kozak sequence for expression in Xenopus
BBa_K1154006BBa_K1154006 Version 1 (Component)Mating pheromone-induced IGPD and constitutive LDH expression in yeast
BBa_M11402BBa_M11402 Version 1 (Component)5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803
BBa_K2020051BBa_K2020051 Version 1 (Component)wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G
BBa_K2123114BBa_K2123114 Version 1 (Component)Stationary phase promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
PrtDEFBBa_K258007 Version 1 (Component)Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
BBa_K2123116BBa_K2123116 Version 1 (Component)Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.