pSB3K3
pSB3K3 Version 1 (Component)
Low to medium copy BioBrick standard vector

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1053206
BBa_K1053206 Version 1 (Component)
Pconst.- RBS- YF1/FixJ-DT- Pfixk2- HHR*- GFP- DT

YF1-HHR
BBa_K1053205 Version 1 (Component)
Pconst. -RBS- YF1/FixJ-DT- Pfixk2- HHR- GFP- DT

BBa_K741003
BBa_K741003 Version 1 (Component)
pRM-RBS-GFP-T

BBa_J52014
BBa_J52014 Version 1 (Component)
NFkB-dnMyD88-link-rLuc

CspB | RFP
BBa_K525234 Version 1 (Component)
Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS

BBa_K1072017
BBa_K1072017 Version 1 (Component)
pGAL1 + Flag + odr-10 + GFP + ADH1Te + pFUS1 + BFP + ADH1Te

BBa_K1399006
BBa_K1399006 Version 1 (Component)
GFP (mut3b) with LAA-ssrA degradation tag

BBa_K1399008
BBa_K1399008 Version 1 (Component)
GFP (mut3b) with DAS-ssrA degradation tag

BBa_J13062
BBa_J13062 Version 1 (Component)
no_RBS_CFP/B0034.YFP

BBa_J13075
BBa_J13075 Version 1 (Component)
Polycistronic mCherry/YFP

BBa_J23080
BBa_J23080 Version 1 (Component)
b0034 derived RBS to match spacing of J01122

BBa_K821010
BBa_K821010 Version 1 (Component)
PLacI+Native RBS+RFP+Native RBS+GFP+Terminator

BBa_K1766004
BBa_K1766004 Version 1 (Component)
OmpR controlled regulatory circuit producing RFP and GFP

BBa_S03362
BBa_S03362 Version 1 (Component)
LacI input to switch

BBa_K1051104
BBa_K1051104 Version 1 (Component)
VDAC1;targeting peptide to mitochondria outer membrane

BBa_K952003
BBa_K952003 Version 1 (Component)
B0034-YF1-B0034-FixJ-PfixK2-amilGFP

BBa_K133079
BBa_K133079 Version 1 (Component)
CMV-SS-HA-UreB-gyrHP-linker

BBa_K133081
BBa_K133081 Version 1 (Component)
CMV-SS-HA-UreB-gyrEC-linker

BBa_K642000
BBa_K642000 Version 1 (Component)
TetR repressor tagged with yBFP

BBa_K642002
BBa_K642002 Version 1 (Component)
cI repressor tagged with yBFP

BBa_K584002
BBa_K584002 Version 1 (Component)
pLac-Lux hybrid + GFP generator

Switch
BBa_K1185000 Version 1 (Component)
B.subtilis Rod to L-form Switch

BBa_K1500002
BBa_K1500002 Version 1 (Component)
IPTG promoter-GFP (IG) backbone

BBa_G00602
BBa_G00602 Version 1 (Component)
Forward primer to C0062

BBa_K1334027
BBa_K1334027 Version 1 (Component)
promoter similar to R0011

BBa_K1954003
BBa_K1954003 Version 1 (Component)
Please go to BBa_K1954001

BBa_K1316005
BBa_K1316005 Version 1 (Component)
ybiJ promoter coupled to mKate2 reporter gene

BBa_K1316003
BBa_K1316003 Version 1 (Component)
yqjF promoter coupled to mKate2 reporter gene

DsbDspBx
BBa_K1659211 Version 1 (Component)
Dispersin B fused with DsbA, with mutation at nucleotide 636 (T to C)

BBa_I730014
BBa_I730014 Version 1 (Component)
pOmpRm-ptetR-LacI repressor-YFP

BBa_K215251
BBa_K215251 Version 1 (Component)
Lpp + 5tmr OmpA + GS Linker + TEV site + NheI Site

LytC-Gly-E
BBa_K316045 Version 1 (Component)
LytC - Glycine Linker - Elastase Cleavage Site - AIP - His Tag

LytC-Heli-
BBa_K316044 Version 1 (Component)
LytC - Helical Linker - TEV Cleavage Site - AIP - His Tag

LytC-Flex-
BBa_K316046 Version 1 (Component)
LytC - Flexible Linker - Elastase Cleavage Site - AIP - His Tag

LytC-Flex-
BBa_K316043 Version 1 (Component)
LytC - Flexible Linker - TEV Cleavage Site - AIP - His Tag

LytC-Heli-
BBa_K316047 Version 1 (Component)
LytC - Helical Linker - Elastase Cleavage Site - AIP - His Tag

LytC-Gly-T
BBa_K316042 Version 1 (Component)
LytC - Glycine Linker - TEV Cleavage Site - AIP - His Tag

BBa_K215250
BBa_K215250 Version 1 (Component)
Lpp + 1tmr OmpA + GS Linker + TEV site + NheI Site

BBa_J75004
BBa_J75004 Version 1 (Component)
RBS-TetR-Ter-lambdaPr-RBS

BBa_K821011
BBa_K821011 Version 1 (Component)
PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator

BBa_K1634000
BBa_K1634000 Version 1 (Component)
Pmyo2-ChR2-YFP (C.elegans)

BBa_K110020
BBa_K110020 Version 1 (Component)
Venus YFP Yeast Optimized

BBa_K1969016
BBa_K1969016 Version 1 (Component)
The linker sequence between ADH1 promoter and ADRB2 (with His-tag)

BBa_K511101
BBa_K511101 Version 1 (Component)
EYFP-FF4x4 MammoBlock

BBa_K102952
BBa_K102952 Version 1 (Component)
TA2In anti-sense input to BBa_K102952

BBa_K102962
BBa_K102962 Version 1 (Component)
TA14In anti-sense input to BBa_K102912

BBa_K102954
BBa_K102954 Version 1 (Component)
TA6In anti-sense input to BBa_K102904

BBa_K102959
BBa_K102959 Version 1 (Component)
TA11In anti-sense input to BBa_K102909