SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

pBSEP1
BBa_K090401 Version 1 (Component)
Gram-positive Shuttle Vector for Episomal Expression

BBa_K156030
BBa_K156030 Version 1 (Component)
Genetic attenuator measurement device (template)

BBa_K116002
BBa_K116002 Version 1 (Component)
measurement of NhaA promoter activity

BBa_K090403
BBa_K090403 Version 1 (Component)
Gram-positive Shuttle Vector for Chromosomal Integration

AD default
BBa_K106400 Version 1 (Component)
Default insert for AarI AD acceptor vectors

BBa_J70034
BBa_J70034 Version 1 (Component)
A BioScaffold Part (Uses AarI) for Library Vector Preparation (see Part Design page)

BBa_J70040
BBa_J70040 Version 1 (Component)
A BioScaffold Part (Uses AarI, MabI) for Library Vector Preparation (see Part Design page)

BBa_J70100
BBa_J70100 Version 1 (Component)
Library Vector Test Plasmid for J70040

BBa_J70110
BBa_J70110 Version 1 (Component)
Library Vector Test Plasmid for J70034

BBa_K173025
BBa_K173025 Version 1 (Component)
Plac measurement system

BBa_K233317
BBa_K233317 Version 1 (Component)
rfp measurement device

BBa_J73998
BBa_J73998 Version 1 (Component)
plasmid vector for Rhodobacter sphaeroides

BBa_J73999
BBa_J73999 Version 1 (Component)
plasmid vector for Rhodobacter sphaeroides

BBa_K299000
BBa_K299000 Version 1 (Component)
RBS measurement device J23100 J61100 GFP

BBa_K299001
BBa_K299001 Version 1 (Component)
RBS measurement device J23100 J61101 GFP

BBa_K299002
BBa_K299002 Version 1 (Component)
RBS measurement device J23100 J61107 GFP

BBa_K299003
BBa_K299003 Version 1 (Component)
RBS measurement device J23100 J61117 GFP

BBa_K299004
BBa_K299004 Version 1 (Component)
RBS measurement device J23100 J61127 GFP

BBa_K299005
BBa_K299005 Version 1 (Component)
RBS measurement device J23100 B0030 GFP

BBa_K299006
BBa_K299006 Version 1 (Component)
RBS measurement device J23100 B0031 GFP

BBa_K299007
BBa_K299007 Version 1 (Component)
RBS measurement device J23100 B0032 GFP

BBa_K299008
BBa_K299008 Version 1 (Component)
RBS measurement device J23100 B0033 GFP

BBa_K299009
BBa_K299009 Version 1 (Component)
RBS measurement device J23100 B0034 GFP

BBa_J95030
BBa_J95030 Version 1 (Component)
BioBrick terminator measurement standard for Rhodobacter sphaeroides

BBa_J95031
BBa_J95031 Version 1 (Component)
BioBrick promoter and RBS measurement reference standard for R. sphaeroides