BBa_J70655BBa_J70655 Version 1 (Component)RFP optimized for expression in E. coli and M. florum
BBa_K1400000BBa_K1400000 Version 1 (Component)PTRE(4)GX Dual input promoter. Activation at tetO binding sites, repression at gal4 sites.
BBa_K1400001BBa_K1400001 Version 1 (Component)PTRE(2)GX Dual input promoter. Activation at tetO binding sites, repression at gal4 sites.
BBa_K299509BBa_K299509 Version 1 (Component)Expression Vector pT7+B0034
BBa_K809721BBa_K809721 Version 1 (Component)DLD3 promoter + kozak + hoiln(lambda phage) + ADH1 terminator
BBa_K809710BBa_K809710 Version 1 (Component)GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator
BBa_J13076BBa_J13076 Version 1 (Component)Monocistronic CFP/YFP expression cassette
BBa_K2005051BBa_K2005051 Version 1 (Component)mCherry with T7 expression (oxidation-resistant)
BBa_K546547BBa_K546547 Version 1 (Component)Constitutive (tetR repressible) LacI and RFP expression
BBa_K1323019BBa_K1323019 Version 1 (Component)Hfq expression cassette under a xylose inducible promoter
BBa_K1438010BBa_K1438010 Version 1 (Component)Bacterial Iron Storage Expressor
BBa_K812132BBa_K812132 Version 1 (Component)mCFP with kozak sequence for expression in Xenopus
BBa_K812133BBa_K812133 Version 1 (Component)sfGFP with kozak sequence for expression in Xenopus
BBa_K812130BBa_K812130 Version 1 (Component)Citrine reporter with a Kozak sequence for expression in Xenopus
BBa_K563053BBa_K563053 Version 1 (Component)vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
BBa_K1154006BBa_K1154006 Version 1 (Component)Mating pheromone-induced IGPD and constitutive LDH expression in yeast
pSBBs0KBBa_K823026 Version 1 (Component)pSB<sub>Bs</sub>0K-P<sub>spac</sub> (replicative Bacillus subtilis expression vector; IPTG inducible
BBa_K1650000BBa_K1650000 Version 1 (Component)Constitutive promoter expressing GFP (ILS 2015)
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.