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Showing 1 - 50 of 109651 result(s)
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Public
IBMc188
IBMc188 Version 1 (Unknown)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-2aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-2aa-ECF20(102-193)-T
Public
IBMc417_seq
IBMc417_seq Version 1 (Unknown)

Public
IBMc402
IBMc402 Version 1 (Unknown)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-QLG-M86N-cat-Plux2-B32-M86C-S-mCherry*(193-236)-H6-T
Public
IBMc413
IBMc413 Version 1 (Unknown)
pSEVA321-PBAD(SapI-)-B30-SrpR(1-170)-LRG-M86N-T
Public
IBMc186
IBMc186 Version 1 (Unknown)
pSB4A3(empty)
Public
IBMc419_seq
IBMc419_seq Version 1 (Unknown)

Public
IBMc323_seq
IBMc323_seq Version 1 (Unknown)

Public
IBMc418_seq
IBMc418_seq Version 1 (Unknown)

Public
IBMc407_seq
IBMc407_seq Version 1 (Unknown)

Public
IBMc451_seq
IBMc451_seq Version 1 (Unknown)

Public
IBMc413_seq
IBMc413_seq Version 1 (Unknown)

Public
IBMc370_seq
IBMc370_seq Version 1 (Unknown)

Public
IBMc360
IBMc360 Version 1 (Unknown)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-M86(1-100)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(101-154)-S-mCherry(193-236)-H6-T
Public
IBMc448
IBMc448 Version 1 (Unknown)
pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(157-236)-H6-Ter
Public
IBMc403_seq
IBMc403_seq Version 1 (Unknown)

Public
IBMc479
IBMc479 Version 1 (Unknown)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-173)-5aa-SYNZIP17-Ter
Public
IBMc330
IBMc330 Version 1 (Unknown)
pSEVA171-PSrpR-B30-mScarlet-I-Ter
Public
IBMc468_seq
IBMc468_seq Version 1 (Unknown)

Public
IBMc401_seq
IBMc401_seq Version 1 (Unknown)

Public
IBMc371_seq
IBMc371_seq Version 1 (Unknown)

Public
IBMc388_seq
IBMc388_seq Version 1 (Unknown)

Public
IBMc426_seq
IBMc426_seq Version 1 (Unknown)

Public
IBMc101_seq
IBMc101_seq Version 1 (Unknown)

Public
IBMc071_seq
IBMc071_seq Version 1 (Unknown)

Public
IBMc389_seq
IBMc389_seq Version 1 (Unknown)

Public
IBMc360_seq
IBMc360_seq Version 1 (Unknown)

Public
IBMc176_seq
IBMc176_seq Version 1 (Unknown)

Public
IBMc259_seq
IBMc259_seq Version 1 (Unknown)

Public
Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Unknown)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Public
IBMc190
IBMc190 Version 1 (Unknown)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-10aa-ECF20(102-193)-T
Public
IBMc469
IBMc469 Version 1 (Unknown)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-121)-Ter
Public
IBMc446_seq
IBMc446_seq Version 1 (Unknown)

Public
IBMc394
IBMc394 Version 1 (Unknown)
pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-14aa-FRB-T
Public
IBMc186_seq
IBMc186_seq Version 1 (Unknown)

Public
IBMc329
IBMc329 Version 1 (Unknown)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR-Ter
Public
IBMc325
IBMc325 Version 1 (Unknown)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-37R3-2-S-mCherry*(193-236)-H6-Ter
Public
IBMc423
IBMc423 Version 1 (Unknown)
pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(115-213)-Ter
Public
IBMc447
IBMc447 Version 1 (Unknown)
pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(122-236)-H6-Ter
Public
IBMc477
IBMc477 Version 1 (Unknown)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-121)-5aa-SYNZIP17-Ter
Public
IBMc308
IBMc308 Version 1 (Unknown)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-6)-BsaI-BsaI-M86(149-154)-S-mCherry*(193-236)-H6-T
Public
IBMc399
IBMc399 Version 1 (Unknown)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-188)-KNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(189-236)-H6-T
Public
IBMc454
IBMc454 Version 1 (Unknown)
pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR-HA-Ter
Public
IBMc226_seq
IBMc226_seq Version 1 (Unknown)

Public
IBMc386_seq
IBMc386_seq Version 1 (Unknown)

Public
IBMc228_seq
IBMc228_seq Version 1 (Unknown)

Public
IBMc326_seq
IBMc326_seq Version 1 (Unknown)

Public
IBMc483
IBMc483 Version 1 (Unknown)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-192)-5aa-SYNZIP17-Ter
Public
IBMc373_seq
IBMc373_seq Version 1 (Unknown)

Public
IBMc078
IBMc078 Version 1 (Unknown)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-27)-BsaI-BsaI-mCherry*(218-236)-T
Public
IBMc472
IBMc472 Version 1 (Unknown)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-176)-Ter
Showing 1 - 50 of 109651 result(s)
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