scar
BBa_J107060 Version 1 (Component)
XbaI/NheI mixed site without the initial CT nucleotides

BBa_I13998
BBa_I13998 Version 1 (Component)
temporary (DE -- delete at some future point as needed)

BBa_K1065309
BBa_K1065309 Version 1 (Component)
light regulated circuit producing amilGFP+EFE at dark

BBa_K2097000
BBa_K2097000 Version 1 (Component)
CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.

BBa_K175050
BBa_K175050 Version 1 (Component)
aTc inducible GFP-LVA generator with integrated I-SceI RS + I-SceI RS + TetR generator

BBa_K861169
BBa_K861169 Version 1 (Component)
Indirect regulatory device, activated at high glucose concentration

BBa_J58120
BBa_J58120 Version 1 (Component)
OmpR-P detector with a RFP pulse generator at intermediate concentrations

BBa_K1641004
BBa_K1641004 Version 1 (Component)
Fusion protein of Cre::EGFP::ssra-tag, with RBS at beginning

IBMc360
IBMc360 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-M86(1-100)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(101-154)-S-mCherry(193-236)-H6-T

BBa_J04606
BBa_J04606 Version 1 (Component)
Antiswitch repressing Biobrick coding sequence at the RBS ("Off" switch)

BBa_M36080
BBa_M36080 Version 1 (Component)
Wild-type miraculin protein with His-tag at C-terminus

IBMc378
IBMc378 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-17)-PI-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(18-154)-S-mCherry*(193-236)-H6-T

IBMc379
IBMc379 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-22)-LG-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(23-154)-S-mCherry*(193-236)-H6-T

IBMc380
IBMc380 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-39)-LD-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(40-154)-S-mCherry*(193-236)-H6-T

BBa_K1641006
BBa_K1641006 Version 1 (Component)
Fusion protein of Vcre::EGFP::ssra-tag, with RBS at beginning

BBa_K1641007
BBa_K1641007 Version 1 (Component)
Fusion protein of Scre::EGFP::ssra-tag, with RBS at beginning

BBa_K564023
BBa_K564023 Version 1 (Component)
atC-->chiX

BBa_K1641009
BBa_K1641009 Version 1 (Component)
Fusion protein of Vika-EGFP-ssra, with RBS at beginning

BBa_K648101
BBa_K648101 Version 1 (Component)
RecA (mutated from RecA1 at amino acid 160 G-->A

(L-C)3
BBa_K365014 Version 1 (Component)
ClpX trimer with built-in linker at N-ter end

BBa_M45689
BBa_M45689 Version 1 (Component)
Constitutive promoter with a retinoic acid response element at the end

BBa_K137035
BBa_K137035 Version 1 (Component)
Device with GFP with (AC)22 repeat after start codon

BBa_K137034
BBa_K137034 Version 1 (Component)
Device with GFP with (AC)20 repeat after start codon

BBa_K806003
BBa_K806003 Version 1 (Component)
SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins

BBa_K1088053
BBa_K1088053 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

BBa_K855006
BBa_K855006 Version 1 (Component)
pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site

BBa_K855005
BBa_K855005 Version 1 (Component)
pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site

BBa_K351007
BBa_K351007 Version 1 (Component)
first Ig-like region of Human basic FGFR with signal peptide for s.pombe surface

BBa_J119408
BBa_J119408 Version 1 (Component)
Pupp promoter mutant - Substitution of C and G to A at 28 and 30

BBa_K202004
BBa_K202004 Version 1 (Component)
Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 3

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1088052
BBa_K1088052 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1178000
BBa_K1178000 Version 1 (Component)
tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon

IodoY RS
BBa_K1416001 Version 1 (Component)
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive

BBa_J107021
BBa_J107021 Version 1 (Component)
aTc sensor (J23106 promoter) with GFP

BBa_K137083
BBa_K137083 Version 1 (Component)
Strong promoter + strong RBS + GFP with (CT)21 repeat after start codon + double terminator

BBa_K137084
BBa_K137084 Version 1 (Component)
Strong promoter + strong RBS + GFP with (CT)20 repeat after start codon + double terminator

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K137021
BBa_K137021 Version 1 (Component)
GFP with (AC)20 repeat after start codon

BBa_K137033
BBa_K137033 Version 1 (Component)
Device with GFP with (AC)21 repeat after start codon

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.