BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K223062
BBa_K223062 Version 1 (Component)
TolC and Hemolysin B, C and D gene complex

BBa_K656008
BBa_K656008 Version 1 (Component)
Yellow fluorescence protein bound to dockerin from C. cellulolyticum

BBa_K656007
BBa_K656007 Version 1 (Component)
Cyan fluorescent protein bound to dockerin of C. thermocellum

ChiPm
BBa_K564015 Version 1 (Component)
ChiP promoter and 5' UTR; RBS mutated AAGAGG-->AGGAGA

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

cinI
BBa_C0076 Version 1 (Component)
autoinducer synthetase

BBa_K2066004
BBa_K2066004 Version 1 (Component)
Tet Monomer C w/ 8 bp spacer for ICA

BBa_K1022121
BBa_K1022121 Version 1 (Component)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT

BBa_K1022120
BBa_K1022120 Version 1 (Component)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2066007
BBa_K2066007 Version 1 (Component)
Tet Monomer C w/ 16 bp spacer for ICA

BBa_K2066013
BBa_K2066013 Version 1 (Component)
Lac Monomer C w/ 16 bp spacer for ICA

BBa_K2066010
BBa_K2066010 Version 1 (Component)
Tet Monomer C w/ 64 bp spacer for ICA

BBa_J100031
BBa_J100031 Version 1 (Component)
Constitutive promoter C on Gene 1 of T7, transcribes RNA Pol.

BBa_J119348
BBa_J119348 Version 1 (Component)
P5 promoter - BD18 C dog - Melamine Deaminase gene in Scaffold 2.0

ompC w/Cad
BBa_M11084 Version 1 (Component)
E coli outer membrane protein C (ompC) with Cadmium Binding protien

BBa_K2170117
BBa_K2170117 Version 1 (Component)
Intermediate of secretion efficiency construct (NanoLuc_StrepTag_PolyA) in RFC[25] C-part

BBa_K305002
BBa_K305002 Version 1 (Component)
Chaplin C (chpDC) with modified signal sequence and sortase recognition motive

BBa_K331024
BBa_K331024 Version 1 (Component)
RBS with N-terminal Oligo Arginine - ECFP Fusion

N-carrier
BBa_K1362053 Version 1 (Component)
Carrier for RFC[105] N-terminal inserts

BBa_K1362057
BBa_K1362057 Version 1 (Component)
NLS as RFC[105] N-terminal insert

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1921003
BBa_K1921003 Version 1 (Component)
PETase MutateM

BBa_K1921002
BBa_K1921002 Version 1 (Component)
PETase MutateJ

pSB6A0
BBa_I751000 Version 1 (Component)
pBR322 (Pst I site mutated)

BBa_K1321004
BBa_K1321004 Version 1 (Component)
N-terminal rfc10_C-terminal rfc25 assembly

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

BBa_K309030
BBa_K309030 Version 1 (Component)
eYFP::unc-54 3'UTR (for use in C. elegans)

DOUBLE
BBa_K1433020 Version 1 (Component)
P-A-T-T-B-C-T-T-D-B0034-GFP-T

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1362173
BBa_K1362173 Version 1 (Component)
Split sfGFP reconstitution N-terminal splicing half

BBa_K2082203
BBa_K2082203 Version 1 (Component)
Mutated fusion protein HA4(R38A):cMyc:RpoZ

BBa_K2082202
BBa_K2082202 Version 1 (Component)
Mutated fusion protein HA4(Y87A):cMyc:RpoZ

BBa_K331022
BBa_K331022 Version 1 (Component)
RBS with N-terminal Oligo Arginine - EYFP Fusion

BBa_K1914005
BBa_K1914005 Version 1 (Component)
pT7 Lysozyme C E.coli codon optimised, signal peptide, flag tag

BBa_K309021
BBa_K309021 Version 1 (Component)
pStr-220::eCFP::3'UTR (for use in C. elegans)

CBDclos
BBa_K863111 Version 1 (Component)
Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)

LAA
BBa_K365006 Version 1 (Component)
LAA tag

BBa_J58117
BBa_J58117 Version 1 (Component)
Mutated OmpR promoter with inverter TetR

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

BBa_K1170003
BBa_K1170003 Version 1 (Component)
p-atp2 in F0F1 ATPase operon from C. glutamicum ATCC 13032

MtrC
BBa_K1748003 Version 1 (Component)
Extracelllular metal reducing respiratory system periplasmic decaheme cytochrome C component MtrC