BBa_K2150029
BBa_K2150029 Version 1 (Component)
pCon+RBS+tetR+DT+pTet+RBS+T7RNAP+Ter+RBS+pT7+tetX-GFP(fusion protein)+DT

BBa_K351000
BBa_K351000 Version 1 (Component)
Signal peptide for cell surface of fission yeast

BBa_K1088053
BBa_K1088053 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1963002
BBa_K1963002 Version 1 (Component)
A template for expression of sRNA fusions to the E. coli micC hairpin

Bm3R1
BBa_K1401000 Version 1 (Component)
TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')

BBa_J70032
BBa_J70032 Version 1 (Component)
A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_K1088052
BBa_K1088052 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_J58009
BBa_J58009 Version 1 (Component)
Fusion protein Trg-EnvZ

BBa_K351005
BBa_K351005 Version 1 (Component)
Normal Human FGF receptor with fission yeast surface signal peptide

BBa_K1648004
BBa_K1648004 Version 1 (Component)
Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane

SISO_CN
BBa_K255002 Version 1 (Component)
pTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator

SISO_LacI
BBa_K255003 Version 1 (Component)
pLac.lacI-cfp(fusion).terminator.pTet.yfp.terminator

BBa_K1582025
BBa_K1582025 Version 1 (Component)
Thc_Cut1+sJanus-m Fusion Protein

BBa_K331021
BBa_K331021 Version 1 (Component)
Catechol 2,3 Dioxygenase with Fusion Suffix

mCherry
BBa_K105010 Version 1 (Component)
mCherry, Yeast optimized for fusion proteins

BBa_K105008
BBa_K105008 Version 1 (Component)
EYFP, yeast optimized for fusion proteins

BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.

BBa_K351004
BBa_K351004 Version 1 (Component)
Modified Human FGF receptor for MPT51 antigen with fission yeast surface signal peptide

BBa_I20293
BBa_I20293 Version 1 (Component)
Constitutive expression of GFP-LacZalpha fusion

BBa_K1413045
BBa_K1413045 Version 1 (Component)
A fusion of Universal Transposon Plasmid and pSB1C3

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

BBa_K371054
BBa_K371054 Version 1 (Component)
MPF(meta-prefix)+[GFP+10*GS+A] fusion protein+MSF(meta-suffix))

BBa_K774102
BBa_K774102 Version 1 (Component)
Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide usin

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.