BBa_K660616BBa_K660616 Version 1 (Component)pBAD with strong RBS
BBa_K2088007BBa_K2088007 Version 1 (Component)pbaB: a 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain.
BBa_J202015BBa_J202015 Version 1 (Component)PBAD Characterization (Wild-type)
BBa_I721007BBa_I721007 Version 1 (Component)pAraC/BAD>ERBS>LacI>ERBS>TetR>ERBS>mRFP1>Terminator
BBa_I13016BBa_I13016 Version 1 (Component)TetR under Pbad control
BBa_K206004BBa_K206004 Version 1 (Component)RFP generator for testing pBAD wt
BBa_K136015BBa_K136015 Version 1 (Component)pBad/araC - GFP tripart
BBa_K180005BBa_K180005 Version 1 (Component)GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]
BBa_K327003BBa_K327003 Version 1 (Component)pBad controlled lux membrane protein
BBa_I746910BBa_I746910 Version 1 (Component)construction intermediate: pBAD promoter - RBS
BBa_K1053006BBa_K1053006 Version 1 (Component)PBAD-TR(42)-HHR-taR12-DT
BBa_J44013BBa_J44013 Version 1 (Component)HixC-pBAD-HixC-Tetrev-RBSrev-HixC
BBa_K1877001BBa_K1877001 Version 1 (Component)pBAD + RBS + GFP + Single Terminator
BBa_K1075036BBa_K1075036 Version 1 (Component)AraC-pBAD-RBS32-ccdA-ssrA-TT
BBa_K1053005BBa_K1053005 Version 1 (Component)PBAD- TR-HHR*(RBS)-GFPuv-DT
BBa_J01066BBa_J01066 Version 1 (Component)TEST arabinose inducible key 1 [pBad/araC][Key1]
BBa_J24673BBa_J24673 Version 1 (Component)pBAD promoter with lacI coding region
BBa_J44010BBa_J44010 Version 1 (Component)HixC-pBAD-HixC-RBS-TetF-HixC
pSBBs0KBBa_K823026 Version 1 (Component)pSB<sub>Bs</sub>0K-P<sub>spac</sub> (replicative Bacillus subtilis expression vector; IPTG inducible
BBa_I12029BBa_I12029 Version 1 (Component)Test of pBAD/araC promoter (I0500)
BBa_J01119BBa_J01119 Version 1 (Component)TEST [pBad/araC][StrRBS][RFP][DblTerm]
GG100BBa_K2145125 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
GG98BBa_K2145123 Version 1 (Component)This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
BBa_S03638BBa_S03638 Version 1 (Component)HixC-pBad<sub>rev</sub>-HixC : RBS-TetF
BBa_K1075003BBa_K1075003 Version 1 (Component)Promoter(const.)-AraC-Term-pBAD-RBS34 (Arabinose inducable promoter system)
BBa_K199070BBa_K199070 Version 1 (Component)I13453:K199016 Pbad promotor with the suppressor tRNA of CUAGC
BBa_S03674BBa_S03674 Version 1 (Component)(-1,2) HixC-pBad<sub>rev</sub>-HixC : RBS-TetF-HixC
BBa_I2031BBa_I2031 Version 1 (Component)GFP expression device controlled by an arabinose inducible pBAD promoter
BBa_K199071BBa_K199071 Version 1 (Component)I13453:K199014: Pbad promomtor with the suppressor tRNA of the codon AGGAC
BBa_I720010BBa_I720010 Version 1 (Component)Ara landing pad (pBBLP 8)
BBa_K323164BBa_K323164 Version 1 (Component)VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5
BBa_K323163BBa_K323163 Version 1 (Component)VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.