BBa_K176172
BBa_K176172 Version 1 (Component)
Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->GFP

BBa_K176168
BBa_K176168 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA

BBa_K176187
BBa_K176187 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.04->luxR+pLux/Tet->ccdB(weak RBS)

BBa_K176131
BBa_K176131 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)

BBa_K176185
BBa_K176185 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.15->luxR+pLux/Tet->ccdB(weak RBS)

BBa_K176090
BBa_K176090 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176186
BBa_K176186 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.15->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176132
BBa_K176132 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176188
BBa_K176188 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.04->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K176115
BBa_K176115 Version 1 (Component)
Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)

BBa_K176169
BBa_K176169 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)

BBa_K176091
BBa_K176091 Version 1 (Component)
Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K2150027
BBa_K2150027 Version 1 (Component)
pCon+RBS+tetR+DT+pT7+pTet+RBS+tetX-GFP(fusion protein)+RBS+T7RNAP+DT

BBa_K176170
BBa_K176170 Version 1 (Component)
TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)

BBa_K2150029
BBa_K2150029 Version 1 (Component)
pCon+RBS+tetR+DT+pTet+RBS+T7RNAP+Ter+RBS+pT7+tetX-GFP(fusion protein)+DT

BBa_K1375022
BBa_K1375022 Version 1 (Component)
promoter+RBS+araC+ DT+pBAD+RBS+Cinr+RBS+CinI+DT+Plac+RBS+LuxI+DT+Pcon+RBS+LacI

BBa_K1375003
BBa_K1375003 Version 1 (Component)
Pcon+RBS+mRFP+DT

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.