BBa_J04795
BBa_J04795 Version 1 (Component)
Riboswitch designed to turn "ON" a protein

BBa_K611017
BBa_K611017 Version 1 (Component)
cI Lambda Repressor and Promoter Wild Type Control

BBa_I763003
BBa_I763003 Version 1 (Component)
GFP coding device switched on by IPTG

placIQ RBS
BBa_K193604 Version 1 (Component)
GFP behind a constitutive promoter (placIQ) on pSB4A5

BBa_K1053209
BBa_K1053209 Version 1 (Component)
Pconst.- RBS-YF1/FixJ-DT- Pfixk2- taRNA- DT- Pconst.- HHR*- GFP- DT

BBa_I759033
BBa_I759033 Version 1 (Component)
cis1-repressed, tet-regulated YFP

BBa_I759045
BBa_I759045 Version 1 (Component)
cis7-repressed, tet-regulated YFP

BBa_I759043
BBa_I759043 Version 1 (Component)
cis6-repressed, tet-regulated YFP

BBa_I759037
BBa_I759037 Version 1 (Component)
cis3-repressed, tet-regulated YFP

BBa_I759047
BBa_I759047 Version 1 (Component)
cis8-repressed, tet-regulated YFP

BBa_I759041
BBa_I759041 Version 1 (Component)
cis5-repressed, tet-regulated YFP

BBa_I759035
BBa_I759035 Version 1 (Component)
cis2-repressed, tet-regulated YFP

BBa_K327015
BBa_K327015 Version 1 (Component)
Lux activated, C1lam repressed switch

placIQ RBS
BBa_K193601 Version 1 (Component)
Constitutive Promoter (placIQ ) + RBS + melA on Low copy vector(pSB6A1)

BBa_K395102
BBa_K395102 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)

BBa_K395103
BBa_K395103 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.