BBa_M11405
BBa_M11405 Version 1 (Component)
Type 1 promoter of petBD operon in Synechocystis sp. PCC 6803

BBa_J70342
BBa_J70342 Version 1 (Component)
J70315psri.f.1: J70315 psri forward part for pcr of S03621 (single stranded)

BBa_J70343
BBa_J70343 Version 1 (Component)
J70315psri.r.1: J70315 psri reverse part for pcr of S03621 (single stranded)

BBa_M11403
BBa_M11403 Version 1 (Component)
Type 1 promoter of the cpcBA operon in Synechocystis sp. PCC 6803

BBa_K1124123
BBa_K1124123 Version 1 (Component)
inverse PCR template for creating new sRNA (plambda-micC sRNA scaffold-terminator)

BBa_K390029
BBa_K390029 Version 1 (Component)
GFP with 5'UTR and consensus RBS from Synechocystis sp. PCC 6803

BBa_K390028
BBa_K390028 Version 1 (Component)
GFP with 5'UTR and native RBS from Synechocystis sp. PCC 6803

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_M11409
BBa_M11409 Version 1 (Component)
Promoter of pglnBP2 gene in Synechocystis sp. PCC 6803.

BBa_M11407
BBa_M11407 Version 1 (Component)
Type 1 promoter of hspA gene in Synechocystis sp. PCC 6803

BBa_M11408
BBa_M11408 Version 1 (Component)
Type 1 promoter of sigA gene in Synechocystis sp. PCC 6803

BBa_M11404
BBa_M11404 Version 1 (Component)
Type 1 promoter of psbA2 gene in Synechocystis sp. PCC 6803

BBa_M11402
BBa_M11402 Version 1 (Component)
5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.