BBa_J06955BBa_J06955 Version 1 (Component)434 cI/lambda cI857 (Lambda II) RS flip-flop
BBa_J06963BBa_J06963 Version 1 (Component)434 cI/lambda cI RS flip-flop with EYFP
BBa_J06951BBa_J06951 Version 1 (Component)Lambda cI/LacI ts (mut 241) RS flip-flop
CsgDBBa_K1019001 Version 1 (Component)CsgD: positive regulator of curlin genes
BBa_J06964BBa_J06964 Version 1 (Component)434 cI/lambda cI857 (E07700) RS flip-flop with EYFP
BBa_K360115BBa_K360115 Version 1 (Component)Mutated red luciferase based on BBa_I712019
BBa_J06961BBa_J06961 Version 1 (Component)Lambda cI/LacI ts (mut 241) RS flip-flop with EYFP
BBa_J06962BBa_J06962 Version 1 (Component)Lambda cI/LacI ts (mut 265) RS flip-flop with EYFP
BBa_J06965BBa_J06965 Version 1 (Component)434 cI/lambda cI857 (Lambda II) RS flip-flop with EYFP
BBa_K892000BBa_K892000 Version 1 (Component)Red light responsive luciferase
BBa_K133039BBa_K133039 Version 1 (Component)T7-CF-UREB-RGD-Histop
BBa_K581003BBa_K581003 Version 1 (Component)SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)
BBa_K2123116BBa_K2123116 Version 1 (Component)Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)
BBa_K549004BBa_K549004 Version 1 (Component)LacI promotor fused with the iron dependent regulator fur
BBa_I741016BBa_I741016 Version 1 (Component)Reverse Total XylR Transcriptional Regulator Left Facing (I741011 reverse complement
BBa_K133037BBa_K133037 Version 1 (Component)T7-CF213-UB33-CF215-RGD-Histop
BBa_K892999BBa_K892999 Version 1 (Component)red light responsive inverter driving luciferase
BBa_K133020BBa_K133020 Version 1 (Component)CMV-CF213-multiHP-CF215-RGD-Histop (term.)
BBa_K133023BBa_K133023 Version 1 (Component)CMV-ss-CF213-multiHP-CF215-RGD-Histop (term)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.