BBa_K1933201
BBa_K1933201 Version 1 (Component)
constitutive expression of anti-Norovirus GII.4 scFv fused to BclA with 6xHis tag

BBa_K1088002
BBa_K1088002 Version 1 (Component)
Reporter system to quantify protein expression of B. sub 1-deoxyxylulose-5-phosphate synthase (Dxs)

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

pLacIQ+eGF
BBa_K193207 Version 1 (Component)
TmTHP with promotor for constituitive expression and with EGFP for fusion of Anti-Freeze Protein

T7+eGFP+Tm
BBa_K193208 Version 1 (Component)
TmTHP with promotor for constituitive expression and with EGFP for fusion of Anti-Freeze Protein

BBa_J37017
BBa_J37017 Version 1 (Component)
AiiA Expression Assay (can be used in conjunction with AHL assay to detect activity)

BBa_K2055015
BBa_K2055015 Version 1 (Component)
Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of Fe

BBa_K1124100
BBa_K1124100 Version 1 (Component)
clock device (plux/tet) (+luxI, +tetR, +aiiA, ++sRNA, +amilGFP, +UV reset device(E234K, -LVA)

Digitalizer
Digitalizer_collection Version 1 (Collection)
A genetic device to digitalize gene expression into a sharp on/off signal.

BBa_K1507016
BBa_K1507016 Version 1 (Component)
Fatty acid induced expression auto regulator (pfadBA-B0034-C0051-pfadBA-B0034-C0051-R0051-B0034-E004

BBa_K1633005
BBa_K1633005 Version 1 (Component)
MOR siRNA-3 (siRNA for mouse Mu opioid receptor)

BBa_K1633004
BBa_K1633004 Version 1 (Component)
MOR siRNA-2 (siRNA for mouse Mu opioid receptor)

BBa_K1633003
BBa_K1633003 Version 1 (Component)
MOR siRNA-1 (siRNA for mouse Mu opioid receptor)

Adapter Bi
BBa_K1807015 Version 1 (Component)
This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of

BBa_K1438024
BBa_K1438024 Version 1 (Component)
ATPCS Expression Device

BBa_K1695049
BBa_K1695049 Version 1 (Component)
pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

BBa_K188006
BBa_K188006 Version 1 (Component)
Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_K1361003
BBa_K1361003 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C

BBa_K1650000
BBa_K1650000 Version 1 (Component)
Constitutive promoter expressing GFP (ILS 2015)

BBa_K1361001
BBa_K1361001 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively weak expression of CsgA,C

CMV + GFP
BBa_K1852000 Version 1 (Component)
GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report

BBa_J70655
BBa_J70655 Version 1 (Component)
RFP optimized for expression in E. coli and M. florum

BBa_K299509
BBa_K299509 Version 1 (Component)
Expression Vector pT7+B0034

CmsRNA
BBa_K1960102 Version 1 (Component)
sRNA, which can silence chloramphenicol resistance

BBa_K2005051
BBa_K2005051 Version 1 (Component)
mCherry with T7 expression (oxidation-resistant)

BBa_K1510105
BBa_K1510105 Version 1 (Component)
sRNA targets histidine kinase 11 mRNA in S.mutans

BBa_K546547
BBa_K546547 Version 1 (Component)
Constitutive (tetR repressible) LacI and RFP expression

BBa_K812132
BBa_K812132 Version 1 (Component)
mCFP with kozak sequence for expression in Xenopus

BBa_K812133
BBa_K812133 Version 1 (Component)
sfGFP with kozak sequence for expression in Xenopus

PchiP-lacZ
BBa_K564002 Version 1 (Component)
Chitoporin fused with lacZ - target for sRNA based regulation

BBa_K812130
BBa_K812130 Version 1 (Component)
Citrine reporter with a Kozak sequence for expression in Xenopus

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

pSBBs0K
BBa_K823026 Version 1 (Component)
pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.