BBa_K2044012
BBa_K2044012 Version 1 (Component)
Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.

BBa_K2044011
BBa_K2044011 Version 1 (Component)
Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.

BBa_K1036003
BBa_K1036003 Version 1 (Component)
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

BBa_M36918
BBa_M36918 Version 1 (Component)
T1 from E. coli rrnB

BBa_K923004
BBa_K923004 Version 1 (Component)
T7 Terminator from pET14 Plasmid

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_S03250
BBa_S03250 Version 1 (Component)
Intermediate part from assembly 317

BBa_S03251
BBa_S03251 Version 1 (Component)
Intermediate part from assembly 317

BBa_S01639
BBa_S01639 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01522
BBa_S01522 Version 1 (Component)
Intermediate part from assembly 240

T25 domain
BBa_K1088056 Version 1 (Component)
T25 domain of CyaA from Bordetella pertussis

BBa_S01276
BBa_S01276 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01412
BBa_S01412 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01280
BBa_S01280 Version 1 (Component)
Intermediate part from assembly 236

BBa_K1968010
BBa_K1968010 Version 1 (Component)
PgdaA constituve promoter from Aspergillus niger

BBa_S03253
BBa_S03253 Version 1 (Component)
Intermediate part from assembly 317

BBa_S01638
BBa_S01638 Version 1 (Component)
Intermediate part from assembly 240

BBa_K1373005
BBa_K1373005 Version 1 (Component)
oprF gene coding porin from Pseudomonas Aeruginosa PAO1

BBa_K165101
BBa_K165101 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*

BBa_S01927
BBa_S01927 Version 1 (Component)
Intermediate part from assembly 283

BBa_S01368
BBa_S01368 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01370
BBa_S01370 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01369
BBa_S01369 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01896
BBa_S01896 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01419
BBa_S01419 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01371
BBa_S01371 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01535
BBa_S01535 Version 1 (Component)
Intermediate part from assembly 240

BBa_K1447002
BBa_K1447002 Version 1 (Component)
Epitope 2 from Ara h 1

BBa_S01997
BBa_S01997 Version 1 (Component)
Intermediate part from assembly 240

BBa_S02002
BBa_S02002 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01907
BBa_S01907 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01936
BBa_S01936 Version 1 (Component)
Intermediate part from assembly 240

BBa_S02107
BBa_S02107 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01996
BBa_S01996 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01376
BBa_S01376 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01379
BBa_S01379 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01663
BBa_S01663 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01660
BBa_S01660 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01898
BBa_S01898 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01381
BBa_S01381 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01830
BBa_S01830 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01845
BBa_S01845 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01380
BBa_S01380 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01377
BBa_S01377 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01842
BBa_S01842 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01383
BBa_S01383 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01843
BBa_S01843 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01378
BBa_S01378 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01826
BBa_S01826 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01382
BBa_S01382 Version 1 (Component)
Intermediate part from assembly 239