BBa_M31541
BBa_M31541 Version 1 (Component)
This is the M13K07 genome refactored between the Hap1 and Bam1 Sites

His6
BBa_K1362431 Version 1 (Component)
N-terminal His6 (C-Intein)

BBa_K112212
BBa_K112212 Version 1 (Component)
{rbs.int!} The integrase gene with native rbs site and stop codon, BBb format

BBa_J61117
BBa_J61117 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_K1676079
BBa_K1676079 Version 1 (Component)
Mutant 3 of LDH and wildtype LDP

BBa_K855006
BBa_K855006 Version 1 (Component)
pvdQ gene with a silent mutation at 1494 bp to remove the internal PstI site

BBa_K855005
BBa_K855005 Version 1 (Component)
pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site

BBa_I737007
BBa_I737007 Version 1 (Component)
Constitutive expression of all the accessory parts needed for our plasmid

BBa_K404107
BBa_K404107 Version 1 (Component)
Beta-Globin-Intron

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_I766216
BBa_I766216 Version 1 (Component)
Combinatorial Cloning B Site

BBa_K1189026
BBa_K1189026 Version 1 (Component)
Linker

BBa_K1636002
BBa_K1636002 Version 1 (Component)
Linker

BBa_J580003
BBa_J580003 Version 1 (Component)
Cate and Arnau testing the registry

Binding
BBa_K1378007 Version 1 (Component)
INPNC-MVN & GFP (the Binding Plasmid)

BBa_K106017
BBa_K106017 Version 1 (Component)
AarI "D" site scar sequence

BBa_K1583012
BBa_K1583012 Version 1 (Component)
Linker in fusion protein

BBa_K1906007
BBa_K1906007 Version 1 (Component)
Intron + Kana

BBa_K346059
BBa_K346059 Version 1 (Component)
PmerT promoter mutant 3

BBa_K1963002
BBa_K1963002 Version 1 (Component)
A template for expression of sRNA fusions to the E. coli micC hairpin

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

SBOL Compliant Software
SBOLCompliantSoftware_collection Version 1 (Collection)
A collection of software that supports the Synthetic Biology Open Language (SBOL) standard

BBa_K1486003
BBa_K1486003 Version 1 (Component)
Flexible linker 2x (GGGS)

BBa_K1778000
BBa_K1778000 Version 1 (Component)
CYC1TATA sequence is derived from the TATA box secific sequence of yeast Saccharomyces cerevisiae

BBa_J52223
BBa_J52223 Version 1 (Component)
rluc-linker-PEST191

BBa_K323084
BBa_K323084 Version 1 (Component)
linker sequence [GGSGGGSGGS]

BBa_K1084003
BBa_K1084003 Version 1 (Component)
promoter 3

BBa_K300084
BBa_K300084 Version 1 (Component)
Phasins with intein downstream (separated by a flexible protein domain linker)

BBa_M36745
BBa_M36745 Version 1 (Component)
The sensor codes for AraC, a transcription factor, which represses the operon.Arabinose induces tran

GeB
BBa_K411102 Version 1 (Component)
GFP_split_B + Linker + eIF4A_split_B

TALE3
BBa_K1861701 Version 1 (Component)
TALE that binds the sequence T CCGACTCGCTGT

TALE1
BBa_K1861501 Version 1 (Component)
TALE that binds the sequence T CTCCACCCCATT

TALE2
BBa_K1861601 Version 1 (Component)
TALE that binds the sequence T GTCGTAACAACT

c-di-AMP
BBa_K1045002 Version 1 (Component)
CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch

BBa_J100042
BBa_J100042 Version 1 (Component)
LuxI with 3 clause 3-SAT problem

BBa_K2122000
BBa_K2122000 Version 1 (Component)
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter

BBa_K1051205
BBa_K1051205 Version 1 (Component)
The RFP with degradation tag

BBa_K1316011
BBa_K1316011 Version 1 (Component)
pFAB640 regulating the ccmAH cluster

BBa_K2055016
BBa_K2055016 Version 1 (Component)
Part designed for verification and characterization of the FUR/LacI inverter

BBa_K1777018
BBa_K1777018 Version 1 (Component)
flexible linker

BBa_K1182011
BBa_K1182011 Version 1 (Component)
Loxp511 linker

BBa_K404309
BBa_K404309 Version 1 (Component)
SEG-Linker

BBa_K404310
BBa_K404310 Version 1 (Component)
GSAT-Linker

BBa_K2082210
BBa_K2082210 Version 1 (Component)
Optimized lacZ promoter with binding site of regulatory protein cI of phage 434

xis
BBa_K112205 Version 1 (Component)
{rbs.xis!} The xis gene with native rbs site and stop codon; assembly standard 21

RFC105 NC
BBa_K1362417 Version 1 (Component)
C-terminal GG-linker overhang GG<u>TGGT</u>=Gly+Gly RFC[105] overhang NC

BBa_K730000
BBa_K730000 Version 1 (Component)
The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide.

BBa_J176052
BBa_J176052 Version 1 (Component)
miRNA CFP-reporter 3

ReA
BBa_K411103 Version 1 (Component)
RFP_split_A + Linker + eIF4A_split_A

BBa_K346089
BBa_K346089 Version 1 (Component)
T7 promoter(BBa_I719005)+MerT+MerP+MerC(the facilitation module of mercury absorption)