BBa_K1059004
BBa_K1059004 Version 1 (Component)
Its transcript can prevent the mRNA itself from being degraded by exonuclease.

BBa_K1650037
BBa_K1650037 Version 1 (Component)
SpyCatcher

BBa_K112805
BBa_K112805 Version 1 (Component)
[T4 holin]

BBa_K1918100
BBa_K1918100 Version 1 (Component)
TRE-Kozak-EBFP2N(1-154)

BBa_M1353
BBa_M1353 Version 1 (Component)
thl + sigA promoter/consensus RBS

BBa_K142008
BBa_K142008 Version 1 (Component)
lacI IS mutant R197A with RBS and terminator

BBa_K142010
BBa_K142010 Version 1 (Component)
lacI IS mutant T276A with RBS and terminator

BBa_K142009
BBa_K142009 Version 1 (Component)
lacI IS mutant R197F with RBS and terminator

ARL4A0604
BBa_I732115 Version 1 (Component)
ARL4A0604

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1321011
BBa_K1321011 Version 1 (Component)
SmtA metallothionein in Freiburg format (RFC[25])

BBa_I766216
BBa_I766216 Version 1 (Component)
Combinatorial Cloning B Site

BBa_J58012
BBa_J58012 Version 1 (Component)
Promoter activated synergistically by cI and CRP with the reporter RFP

BBa_M34014
BBa_M34014 Version 1 (Component)
Nucleotide sequence of a cDNA clone of saxiphilin from the liver of Rana Catesbeiana

BBa_K195409
BBa_K195409 Version 1 (Component)
This part is composed of RBS,PelB,F10scFv and C-IgAP

BBa_K902034
BBa_K902034 Version 1 (Component)
CarAd Coding Sequence

BBa_M36451
BBa_M36451 Version 1 (Component)
Holin Coding Sequence

BBa_M36453
BBa_M36453 Version 1 (Component)
Endolysin Coding Sequence

BBa_K896983
BBa_K896983 Version 1 (Component)
this is a gene about a T cell receptor

BBa_K310010
BBa_K310010 Version 1 (Component)
GolS: Transcription factor that is activated upon binding gold

BBa_K1778006
BBa_K1778006 Version 1 (Component)
Kana-His is a selective marker gene in experiment.

<-BsaI
BBa_K1362447 Version 1 (Component)
BsaI reverse restriction site for RFC[???] cloning (shortened by one C)

BBa_K094100
BBa_K094100 Version 1 (Component)
cheZ gene

BBa_K391001
BBa_K391001 Version 1 (Component)
Construct for characterization of P2 activity in the presence of AIP.

BBa_K391000
BBa_K391000 Version 1 (Component)
Construct for characterization of P2 activity in the presence of AIP.

BBa_I737007
BBa_I737007 Version 1 (Component)
Constitutive expression of all the accessory parts needed for our plasmid

CmR
BBa_J31005 Version 1 (Component)
chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]

PLac-CI-La
BBa_I763019 Version 1 (Component)
CI and LacY coding device switched on by IPTG with GFP as a reporter

BBa_K313026
BBa_K313026 Version 1 (Component)
as RNA target2

BBa_I763021
BBa_I763021 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

pCMV-ECFP-
BBa_I763022 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

pTetR-LacI
BBa_I763035 Version 1 (Component)
LacI coding device regulated by pTetR with GFP as a reporter

GFP-AID
BBa_K812110 Version 1 (Component)
GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid

BBa_J119315
BBa_J119315 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A and B)

BBa_K078002
BBa_K078002 Version 1 (Component)
A fragment that encodes an enzyme involed in dioxin degradation, the third step

BBa_K1180002
BBa_K1180002 Version 1 (Component)
fusion protein of lamp 2b and RVG for brain targeting of the exosome

BBa_K1363005
BBa_K1363005 Version 1 (Component)
R0082-HfiA the HfiA protein express passway which can be regulated by light

BBa_K1963002
BBa_K1963002 Version 1 (Component)
A template for expression of sRNA fusions to the E. coli micC hairpin

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

BBa_K078004
BBa_K078004 Version 1 (Component)
2&#65292;2???&#65292;3-Trihydroxybiphenyl 1,2-dioxygenase. The second step enzyme in dixon degradati

SBOL Compliant Software
SBOLCompliantSoftware_collection Version 1 (Collection)
A collection of software that supports the Synthetic Biology Open Language (SBOL) standard

BBa_S03723
BBa_S03723 Version 1 (Component)
RBS-Kan-RBS-Tet-RBS-RFP (pSB1A2)

BBa_K2066538
BBa_K2066538 Version 1 (Component)
Synthetic Enhancer Project: NRII2302 Mutant Coding

BBa_K1323012
BBa_K1323012 Version 1 (Component)
Spc - Spectinomycin Resistance Gene Coding Sequence

BBa_K1323013
BBa_K1323013 Version 1 (Component)
aadD - Kanamycin Resistance Gene Coding Sequence

pBAD-GFP
BBa_K845000 Version 1 (Component)
iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).

motB
BBa_K1463750 Version 1 (Component)
MotB

<- BsaI
BBa_K1362427 Version 1 (Component)
BsaI reverse restriction site for RFC[???] cloning (shortened by one C)

BBa_K912005
BBa_K912005 Version 1 (Component)
ClC-ec1 chloride antiporter

BBa_K177010
BBa_K177010 Version 1 (Component)
invasine locus from yersinia