BBa_K1361001
BBa_K1361001 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively weak expression of CsgA,C

BBa_K2044000
BBa_K2044000 Version 1 (Component)
Based on our project, <2-6-8> is the optimal pathway scheme from Site No. 2 to Site No. 8

BBa_K364332
BBa_K364332 Version 1 (Component)
TRE-Gal4-PXR-PolyA in PSB1A3 (expression vector)

BBa_K364333
BBa_K364333 Version 1 (Component)
TRE-Gal4-EcR-PolyA in PSB1A3 (expression vector)

BBa_K1116003
BBa_K1116003 Version 1 (Component)
Tre-LacI with miR-FF5 and miR-FF3 target

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_K368007
BBa_K368007 Version 1 (Component)
tet-on Promoter (prev TRE) + TEV recogn. site+ N-degron+SF3b155

TriI
BBa_K1152005 Version 1 (Component)
Expression cassette for NRPS synthesizing a Phe-Orn-Leu-Tripeptide

BBa_K368003
BBa_K368003 Version 1 (Component)
tet-on Promoter (prev TRE) + attB + Human Bak + SV40PA

"Scissors"
BBa_K1001755 Version 1 (Component)
AraC generator then Lambda promoter controlling RFP production, "Scissors"

BBa_I745012
BBa_I745012 Version 1 (Component)
pLV-TH-siGata3-3'

BBa_K511911
BBa_K511911 Version 1 (Component)
Inducible rtTA Transactivator Generator (TRE-Tight-rtTA3) MammoBlock Device

BBa_K511909
BBa_K511909 Version 1 (Component)
Inducible LacI Repressor Generator (TRE-Tight-LacI) MammoBlock Device

BBa_K1947028
BBa_K1947028 Version 1 (Component)
Improve the protein purification.

BBa_K1947027
BBa_K1947027 Version 1 (Component)
Improve the protein purification.

BBa_K1947026
BBa_K1947026 Version 1 (Component)
Improve the protein purification.

Superplasm
BBa_K076013 Version 1 (Component)
pLV-TRE-Lbx1-T2A-GLRA1-P2A-D5R-Ubc-Bla

TNY2AKUB
BBa_K076000 Version 1 (Component)
pLV-TRE-Ngn1-linker-EYFP-2A-mKate-Ubc-Bla

BBa_K1072019
BBa_K1072019 Version 1 (Component)
pGAL1 + Brho + Flag + odr-10 + GFP +ADH1 Te + pFUS1 + BFP + ADH1 Te

BBa_K1363100
BBa_K1363100 Version 1 (Component)
a rna part sensetive to th

BBa_K511816
BBa_K511816 Version 1 (Component)
Inducible Blue Fluorescent Protein Generator (TRE-Tight-EBFP2) MammoBlock Device

BBa_K511815
BBa_K511815 Version 1 (Component)
Inducible Red Fluorescent Protein Generator (TRE-Tight-mKate) MammoBlock Device

Q04510+RFP
BBa_K131018 Version 1 (Component)
Intermediate for the Response circuit

BBa_K1983016
BBa_K1983016 Version 1 (Component)
PheP under constitutive promoter and tRNA-Phe under DH10B promoter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K1072016
BBa_K1072016 Version 1 (Component)
pGAL1 + Brho + EGFP + ADH1 Te

BBa_K1351017
BBa_K1351017 Version 1 (Component)
SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>

BBa_K1072013
BBa_K1072013 Version 1 (Component)
pGAL1+ Flag + odr-10 + GFP + ADH1 Te

BBa_K511817
BBa_K511817 Version 1 (Component)
Inducible Yellow Fluorescent Protein Generator (TRE-Tight-EYFP-FF4x4) MammoBlock Device

BBa_K511906
BBa_K511906 Version 1 (Component)
Inducible CI434-VP16 Transactivator Generator (TRE-Tight-CI434-VP16) MammoBlock Device

BBa_K511910
BBa_K511910 Version 1 (Component)
Inducible LacI-KRAB Repressor Generator (TRE-Tight-LacI-KRAB) MammoBlock Device

BBa_K511908
BBa_K511908 Version 1 (Component)
Inducible Gal4-VP16 Transactivator Generator (TRE-Tight-Gal4-VP16) MammoBlock Device

BBa_K511814
BBa_K511814 Version 1 (Component)
Inducible Red Fluorescent Ligand Generator (TRE-Tight-Delta-mCherry) MammoBlock Device

BBa_K511907
BBa_K511907 Version 1 (Component)
Inducible Mnt-VP16 Transactivator Generator (TRE-Tight-Mnt-VP16) MammoBlock Device

BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.

BBa_K1179078
BBa_K1179078 Version 1 (Component)
TRE-tight

BBa_M36914
BBa_M36914 Version 1 (Component)
Codes for the benzylalcohol acetyltransferase enzyme

BBa_K1072014
BBa_K1072014 Version 1 (Component)
pGAL1+ Flag + Brho + odr-10 + GFP + ADH1 Te

BBa_K1431101
BBa_K1431101 Version 1 (Component)
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA

BBa_K1947017
BBa_K1947017 Version 1 (Component)
We verify the expression effect of Mms13.

BBa_K511818
BBa_K511818 Version 1 (Component)
Inducible Notch-Gal4-ESN Receptor Generator (TRE-Tight-Notch-Gal4-ESN) MammoBlock Device

BBa_K1051262
BBa_K1051262 Version 1 (Component)
The measurement pathway of degradation tag K1051208.

BBa_K1051260
BBa_K1051260 Version 1 (Component)
The measurement pathway of degradation tag K1051206.

BBa_K1051261
BBa_K1051261 Version 1 (Component)
The measurement pathway of degradation tag K1051207.

BBa_K1323020
BBa_K1323020 Version 1 (Component)
oriV from the Staphylococcus aureus pSK41 plasmid clone 3