BBa_K2170116
BBa_K2170116 Version 1 (Component)
Intermediate of biotin binding receptor (A3C5_EGFR TMD_mRuby3_StrepTag_PolyA) in RFC[25] C-part

BBa_K323005
BBa_K323005 Version 1 (Component)
CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system

BBa_K2170115
BBa_K2170115 Version 1 (Component)
Intermediate of biotin binding receptor (EGFR TMD_mRuby3_StrepTag_PolyA) in RFC[25] C-part

BBa_K323021
BBa_K323021 Version 1 (Component)
CMV ATG Gli1 link nCFP stop - part of mammalian split/FRET system

BBa_K2170144
BBa_K2170144 Version 1 (Component)
Intermediate of biotin binding receptor (scAvidin_A3C5_EspP_double terminator) in RFC[25] C-part

BBa_K2170122
BBa_K2170122 Version 1 (Component)
Intermediate of biotin binding receptor (EGFR TMD_mRuby3_StrepTag) in RFC[25] C-part

BBa_K2170123
BBa_K2170123 Version 1 (Component)
Intermediate of signal peptide testing construct (mRuby3_StrepTag) in RFC[25] N-part

BBa_K2170124
BBa_K2170124 Version 1 (Component)
Intermediate of signal peptide testing construct (mRuby3_StrepTag_polyA) in RFC[25] N-part

BBa_K2170142
BBa_K2170142 Version 1 (Component)
Intermediate of biotin binding receptor (NanoLuc_StrepTag_double terminator) in RFC[25] C-part

BBa_J70342
BBa_J70342 Version 1 (Component)
J70315psri.f.1: J70315 psri forward part for pcr of S03621 (single stranded)

BBa_K2170143
BBa_K2170143 Version 1 (Component)
Intermediate of biotin binding receptor (BAP_NanoLuc_A3C5_EspP_double terminator) in RFC[25] C-part

BBa_J70343
BBa_J70343 Version 1 (Component)
J70315psri.r.1: J70315 psri reverse part for pcr of S03621 (single stranded)

SbpA | CFP
BBa_K525406 Version 1 (Component)
Fusion Protein of S-Layer SbpA and mCerulean

SgsE | YFP
BBa_K525305 Version 1 (Component)
Fusion Protein of S-Layer SgsE and mCitrine

BBa_K1315002
BBa_K1315002 Version 1 (Component)
The <i>rpfC</i> gene from <i>Xanthomonas campestris</i>

BBa_K362003
BBa_K362003 Version 1 (Component)
oxyR,this promoter is controlled by CRP-cAMP

BBa_K362000
BBa_K362000 Version 1 (Component)
AcrAB,this promoter is controlled by SoxS protein.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_E041010
BBa_E041010 Version 1 (Component)
Intermediate part from assembly 317

BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_K2044006
BBa_K2044006 Version 1 (Component)
Based on our project,<3,6> is the direct pathway from Site No.3 to Site No.6 in the map we design.

BBa_K2044004
BBa_K2044004 Version 1 (Component)
Based on our project, <2,4> is the direct pathway from Site No.2 to Site No.4 in the map we design.

BBa_K2044009
BBa_K2044009 Version 1 (Component)
Based on our project, <4,8> is the direct pathway from Site No.4 to Site No.8 in the map we design.

BBa_K2044014
BBa_K2044014 Version 1 (Component)
Based on our project, <1,4> is the direct pathway from Site No.1 to Site No.4 in the map we design.

BBa_K2044007
BBa_K2044007 Version 1 (Component)
Based on our project, <4,5> is the direct pathway from Site No.4 to Site No.5 in the map we design.

BBa_K2044013
BBa_K2044013 Version 1 (Component)
Based on our project,<7,8> is the direct pathway from Site No.7 to Site No.8 in the map we design.

BBa_K2044008
BBa_K2044008 Version 1 (Component)
Based on our project, <4,6> is the direct pathway from Site No.4 to Site No.6 in the map we design.

BBa_K2044003
BBa_K2044003 Version 1 (Component)
Based on our project, <2,3> is the direct pathway from Site No.2 to Site No.3 in the map we design.

BBa_K2044002
BBa_K2044002 Version 1 (Component)
Based on our project, <2,1> is the direct pathway from Site No.2 to Site No.1 in the map we design.

BBa_K2044005
BBa_K2044005 Version 1 (Component)
Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.

BBa_K2044012
BBa_K2044012 Version 1 (Component)
Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.

BBa_K2044011
BBa_K2044011 Version 1 (Component)
Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.

ihfA
BBa_K112218 Version 1 (Component)
{rbs.ihfA!} The ihf alpha gene with native rbs and stop codon, BBb format

ihfB
BBa_K112224 Version 1 (Component)
{rbs.ihfB!} The ihf beta gene with native rbs and stop codon, BBb format

BBa_K1532008
BBa_K1532008 Version 1 (Component)
project system we used to slove the SPP

Test
BBa_K294210 Version 1 (Component)
Test part

BBa_J11040
BBa_J11040 Version 1 (Component)
Joseph's Part

BBa_K106677
BBa_K106677 Version 1 (Component)
Ssn8, Aar1 AD part

BBa_K112216
BBa_K112216 Version 1 (Component)
{< ihfA >} The ihf alpha gene without start and stop codons, BBb format

Met-His
BBa_K133035 Version 1 (Component)
Methionine-His affinity tag

BBa_K110016
BBa_K110016 Version 1 (Component)
A-Cell Promoter STE2 (backwards)

BBa_K726003
BBa_K726003 Version 1 (Component)
T7 promoter + lac operator + His tag

BBa_M333334
BBa_M333334 Version 1 (Component)
Produces Beta-carotene from the biochemical input GGPP

BBa_J04606
BBa_J04606 Version 1 (Component)
Antiswitch repressing Biobrick coding sequence at the RBS ("Off" switch)

BBa_K422007
BBa_K422007 Version 1 (Component)
CheY (AarI A-part)

BBa_K1618014
BBa_K1618014 Version 1 (Component)
Plac-THL

BBa_K256026
BBa_K256026 Version 1 (Component)
B0034:CHE