BBa_K1216013BBa_K1216013 Version 1 (Component)BBa_K1216013 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.
BBa_K1216011BBa_K1216011 Version 1 (Component)BBa_K1216007 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.
BBa_K1216010BBa_K1216010 Version 1 (Component)BBa_K1216010 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL. (
Bm3R1BBa_K1401000 Version 1 (Component)TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
BBa_K2044001BBa_K2044001 Version 1 (Component)Based on our project, <2-4-8> is a feasible pathway from Site No. 2 to Site No. 8
BBa_K987001BBa_K987001 Version 1 (Component)This is a composite part which has the function to invert the temperature activation by the part: BB
BBa_K987000BBa_K987000 Version 1 (Component)This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p
BBa_K2044000BBa_K2044000 Version 1 (Component)Based on our project, <2-6-8> is the optimal pathway scheme from Site No. 2 to Site No. 8
BBa_K2044010BBa_K2044010 Version 1 (Component)Based on our project, <5,7> is the direct pathway from Site No.5to Site No.7 in the map we design.
BBa_K2044006BBa_K2044006 Version 1 (Component)Based on our project,<3,6> is the direct pathway from Site No.3 to Site No.6 in the map we design.
BBa_K2044004BBa_K2044004 Version 1 (Component)Based on our project, <2,4> is the direct pathway from Site No.2 to Site No.4 in the map we design.
BBa_K2044009BBa_K2044009 Version 1 (Component)Based on our project, <4,8> is the direct pathway from Site No.4 to Site No.8 in the map we design.
BBa_K2044014BBa_K2044014 Version 1 (Component)Based on our project, <1,4> is the direct pathway from Site No.1 to Site No.4 in the map we design.
BBa_K2044007BBa_K2044007 Version 1 (Component)Based on our project, <4,5> is the direct pathway from Site No.4 to Site No.5 in the map we design.
BBa_K2044013BBa_K2044013 Version 1 (Component)Based on our project,<7,8> is the direct pathway from Site No.7 to Site No.8 in the map we design.
BBa_K2044008BBa_K2044008 Version 1 (Component)Based on our project, <4,6> is the direct pathway from Site No.4 to Site No.6 in the map we design.
BBa_K2044003BBa_K2044003 Version 1 (Component)Based on our project, <2,3> is the direct pathway from Site No.2 to Site No.3 in the map we design.
BBa_K2044002BBa_K2044002 Version 1 (Component)Based on our project, <2,1> is the direct pathway from Site No.2 to Site No.1 in the map we design.
BBa_K2044005BBa_K2044005 Version 1 (Component)Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.
BBa_K2044012BBa_K2044012 Version 1 (Component)Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.
BBa_K2044011BBa_K2044011 Version 1 (Component)Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.
BBa_J580110BBa_J580110 Version 1 (Component)This is my first part
BBa_K783040BBa_K783040 Version 1 (Component)This is a MoClo converted version of BBa_J23110
BBa_K783034BBa_K783034 Version 1 (Component)This is a MoClo converted version of BBa_J23114
BBa_K896986BBa_K896986 Version 1 (Component)this is a gene about a T cell receptor
BBa_K294205BBa_K294205 Version 1 (Component)This is a coding sequence of heat shock protein from E.coli
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_K831011BBa_K831011 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
BBa_K831012BBa_K831012 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.