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IBMc071

IBMc071 Version 1

Component

Created by: Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc071_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
ECF20 expression plasmid. Activity reference in IBM experiment on ECF20

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0033 RFC10 Prefix (full) VR tetR tetR promoter ECK120033736 RFC10 Suffix PBAD(SapI-) p15A origin of replication ECF20 VF2 araC	2714,2724 1494,1515 complement(3489,3508) 98,1291 9,97 3321,3373 3374,3394 2422,2705 3955,4779 2731,3309 1371,1390 complement(1516,2394)	ribosome_entry_site biological_region primer_binding_site CDS promoter terminator biological_region promoter origin_of_replication CDS primer_binding_site CDS

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/trevorho
synbiohub#topLevel	IBMc071/1

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Intein_assisted_Bisection_Mapping

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