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IBMc333

IBMc333 Version 1

Component

Created by: Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR(1-22)-BsaI-BsaI-SrpR(192-213)-Ter

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc333_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR(1-22)-BsaI-BsaI-SrpR(192-213)-Ter
Expression vector to generate ORF insertion library in IBM on SrpR

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0030 VR araC VF2 SrpR(192-213) SrpR(1-22) ECK120033736 spacing sequence PBAD(SapI-) tetR RFC10 Suffix RFC10 Prefix (full) p15A origin of replication tetR promoter	2714,2728 complement(3079,3098) complement(1516,2394) 1371,1390 2833,2899 2735,2802 2911,2963 2810,2825 2422,2705 98,1291 2964,2984 1494,1515 3545,4369 9,97	ribosome_entry_site primer_binding_site CDS primer_binding_site CDS CDS terminator biological_region promoter CDS biological_region biological_region origin_of_replication promoter

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/trevorho
synbiohub#topLevel	IBMc333/1

Member of these Collections

Intein_assisted_Bisection_Mapping

Attachments

Sequence Visualization

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