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IBMc367

IBMc367 Version 1

Component

Created by: Trevor Y. H. Ho,Baojun Wang

pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc367_seq (Version 1)

Description

pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
Staging plasmid for IBM on ECF20

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB1C3
Origin of replication: pMB1
Selection Markers: Chloramphenicol

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
VR cat (BsmBI-) lambda t0 terminator VF2 RFC10 Prefix (full) RFC10 Suffix cat promoter ECF20 rep (pMB1)	complement(1848,1867) complement(221,880) complement(106,200) 992,1011 1110,1131 1694,1714 complement(881,978) 1139,1685 complement(1954,2568)	primer_binding_site CDS terminator primer_binding_site biological_region biological_region promoter CDS origin_of_replication

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/trevorho
synbiohub#topLevel	IBMc367/1

Member of these Collections

Intein_assisted_Bisection_Mapping

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