BBa_B0011

BBa_B0011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_B0011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:20

LuxICDABEG (+/-)



Types
DnaRegion

Roles
terminator

Terminator

Sequences BBa_B0011_sequence (Version 1)

Description

Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.



Notes

In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.



Source

Derived from luxICDABEG operon terminator of Vibrio fischeri AF170104.

Sequence Annotation Location Component / Role(s)
stem_loop
BBa_B0011
13,35
1,46
stem_loop feature/stem_loop
engineered_region feature/BioBrick
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_B0011/1