BBa_B0012

BBa_B0012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_B0012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:20

TE from coliphageT7



Types
DnaRegion

Roles
Terminator

terminator

Sequences BBa_B0012_sequence (Version 1)

Description

Transcription terminator for the E.coli RNA polymerase.

Notes

Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).

Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.

Source

Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146.

Sequence Annotation Location Component / Role(s)
T7 TE
stop
polya
BBa_B0012
8,27
34,34
28,41
1,41
feature/stem_loop stem_loop
stop_codon feature/stop
polyA_site feature/polya
feature/BioBrick engineered_region
igem#experience Fails  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_B0012/1