BBa_B0030

BBa_B0030 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_B0030
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:20

RBS.1 (strong) -- modified from R. Weiss



    • Details

    Types
    DnaRegion

    Roles
    RBS

    ribosome_entry_site

    Sequences BBa_B0030_sequence (Version 1)

    Description

    Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033.

    Notes

    Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis).

    No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.

    Contact info Brian Chow

    Sequence Annotation Location Component / Role(s)
    RBS-1\Strong
    RBS
    BBa_B0030
    		1,15
    8,12
    1,15
    		ribosome_entry_site feature/rbs
    feature/rbs ribosome_entry_site
    feature/BioBrick engineered_region

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_B0030/1
     

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