BBa_B0030

BBa_B0030 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_B0030
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:20

RBS.1 (strong) -- modified from R. Weiss



Types
DnaRegion

Roles
RBS

ribosome_entry_site

Sequences BBa_B0030_sequence (Version 1)

Description

Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033.

Notes

Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis).

No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.

Contact info Brian Chow

Sequence Annotation Location Component / Role(s)
RBS-1\Strong
RBS
BBa_B0030
1,15
8,12
1,15
ribosome_entry_site feature/rbs
feature/rbs ribosome_entry_site
feature/BioBrick engineered_region
igem#experience Works  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_B0030/1