BBa_B0033

BBa_B0033 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_B0033
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:20

RBS.4 (weaker) -- derivative of BBa_0030



Types
DnaRegion

Roles
ribosome_entry_site

RBS

Sequences BBa_B0033_sequence (Version 1)

Description

Weaker RBS based on Ron Weiss thesis. Strengths relative to BBa_B0030, BBa_B0031, BBa_B0032.

Notes

Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis).

No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.

Contact info for this part: Brian Chow

Sequence Annotation Location Component / Role(s)
RBS-4\Weaker
RBS
BBa_B0033
1,11
7,10
1,11
ribosome_entry_site feature/rbs
ribosome_entry_site feature/rbs
engineered_region feature/BioBrick
igem#experience Works  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_B0033/1