BBa_B0033

BBa_B0033 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_B0033
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:20

RBS.4 (weaker) -- derivative of BBa_0030



    • Details

    Types
    DnaRegion

    Roles
    ribosome_entry_site

    RBS

    Sequences BBa_B0033_sequence (Version 1)

    Description

    Weaker RBS based on Ron Weiss thesis. Strengths relative to BBa_B0030, BBa_B0031, BBa_B0032.

    Notes

    Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis).

    No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.

    Contact info for this part: Brian Chow

    Sequence Annotation Location Component / Role(s)
    RBS-4\Weaker
    RBS
    BBa_B0033
    		1,11
    7,10
    1,11
    		ribosome_entry_site feature/rbs
    ribosome_entry_site feature/rbs
    engineered_region feature/BioBrick

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_B0033/1
     

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