TOPO

BBa_B0044 Version 1

Component

Source: http://parts.igem.org/Part:BBa_B0044

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Reshma Shetty

Date created: 2006-02-04 12:00:00

Date modified: 2015-08-31 04:07:20

Topoisomerase I mediated cloning site (reverse)

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_B0044_sequence (Version 1)

Description

Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Nt.BstNB I recognition site	1,5	sequence_feature, feature/misc
Arbitrary sequence (could be any bases)	6,8	sequence_feature, feature/misc
Topoisomerase I recognition site	9,13	feature/misc, sequence_feature
Generated T overhang	9,9	feature/misc, sequence_feature

Other Properties

• igem#experience: Fails
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_B0044/1

Member of these Collections

• //plasmid/component/other
• iGEM Parts Registry