Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Heather Keller
Date created: 2006-09-27 11:00:00
Date modified: 2015-08-31 04:07:21
T7 RBS 0.3 + SapI (rev)
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_B2101_sequence (Version 1) |
Description
This version of the T7 0.3 RBS is designed for construction via Heather Keller's alternative assembly plan using XbaI and SapI for RBS/CDS assembly without a mixed site. Contains the -20 to -1 region of gp0.4 from the wild-type T7 genome. Can also be used with Standard BioBricks assembly, although this will add a 7 amino acid fusion to the N-terminus of the resulting protein.Notes
This RBS is designed to be compatible with Heather Keller's RBS assembly scheme for creating RBS/CDS junctions without a mixed site. For use in this scheme, this part should be cut with XbaI and SapI. It must be used with a coding sequence containing the SapI site at the 5' end. This part is not compatible with CDSs that use a GTG site and must be used with CDSs using an ATG start site.The two bp spacer on the 3' end also allows this part to be used in standard biobricks assembly and is necessary to maintain the reading frame of a downstream CDS. Note that Standard Biobricks Assembly with this part results in the creation of a new ATG start site, and a 7 amino acid fusion at the N terminus of the downstream protein. CDSs with alternative start sites are compatible with this part via Standard Assembly.
This part was inadvertently constructed without the single base pair (G/C) that separates the XbaI site and the 5' end of the part.