Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:23
lacI repressor from E. coli (+LVA)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_C0012_sequence (Version 1) |
Description
Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulatorA rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
Notes
References (unparsed) here:Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00]
References (unparsed) here:
Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00]
Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation.
Incompatible with systems containing LacI, lactose, or IPTG.