lacI

BBa_C0012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_C0012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:23

lacI repressor from E. coli (+LVA)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_C0012_sequence (Version 1)

Description

Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.

A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.



Notes

References (unparsed) here:

Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00]

References (unparsed) here:

Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00]

Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation.

Incompatible with systems containing LacI, lactose, or IPTG.

Source

represillator of Elowitz and Leibler (2000)

Sequence Annotation Location Component / Role(s)
LVA
lacI-LVA
Help:Barcodes
BBa_C0012
1090,1128
1,1128
1129,1153
1,1128
tag feature/tag
CDS feature/cds
feature/barcode engineered_tag
feature/BioBrick engineered_region
igem#experience None  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_C0012/1