aiiA

BBa_C0060 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_C0060
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:23

autoinducer inactivation enzyme from Bacillus
hydrolyzes acetyl homoserine lactone



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_C0060_sequence (Version 1)

Description

Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.



Notes

References (unparsed) here:

Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia
carotovora
, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526

Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491

References (unparsed) here:

Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia
carotovora
, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526

Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491

BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.



Source

AF196486 from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene.
Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia
carotovora
, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).


Sequence Annotation Location Component / Role(s)
start
2
LVA
aiiA
Help:Barcodes
BBa_C0060
1,3
784,789
751,783
1,750
790,814
1,789
feature/start start_codon
feature/stop stop_codon
tag feature/tag
CDS feature/cds
feature/barcode engineered_tag
feature/BioBrick engineered_region
igem#experience None  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_C0060/1